To establish the presence of amphibian and reptile species by cover and their diet in agroecosystems in the Colombian coffee belt, we sampled four farms in the Departamento del Quindío and two in the Departamento del Valle del Cauca (Colombia) from 2006 to 2008. Specimens were captured using both the VES technique and manual capture; each gastrointestinal sample was analyzed to get the type of diet (carnivore, omnivore or herbivore) and the frequency of consumed items (detritus, animal parts, plant material and minerals). Both the sensitivity index (IS) and the dominance index were calculated to find out whether each species belongs to open areas or forest, and to determine niche breadth. Differences between stomach and intestine were analyzed by using a Chi-square test. We captured 376 individuals of 19 species (nine frogs, three lizards, six snakes and one turtle) in crops, pastures, forests and forest of Guadua angustifolia. Centrolenid frogs were found mainly in forests and Guadua, and Rhinella marina, Leptodactylus colombiensis, Lithobates catesbeianus, two species of snakes and a turtle, in open areas. Gastrointestinal contents showed that most of the species are carnivorous. This paper is the first report of the diet of these species in Colombia especially in agroecosystems, being important to compare differences due to habitat transformation.