Etheostoma parvipinne (Goldstripe Darter) is an ecologically enigmatic percid native to lowland streams in the coastal plain of the southeastern United States. Our observations from 6 separate localities across Alabama suggest that Goldstripe Darters seasonally move from resident stream habitats to isolated seepages and vernal pools to spawn. Specifically, at 6 separate sites in Alabama we documented Goldstripe Darters inhabiting and reproducing in isolated seasonal pools and semi-isolated seeps with other vernal pool spawners including mole salamanders (Ambystoma spp.). Notably, some vernal pools used by Goldstripe Darters for reproduction were located >100 m away from the nearest creek with no observed aquatic connection or atop incised stream banks several meters tall. Similar behaviors have been documented in some members of the revised clade Psychromaster and Etheostoma trisella (Trispot Darter), which are listed as special concern, threatened, or endangered by federal and state agencies. While the Goldstripe Darter was previously hypothesized to spawn in seasonal floodplain and spring seepage habitats, we are the first to document this behavior; however, it is not yet clear if this behavior is range wide. Our observations will have implications on the understanding of the evolution of complex reproductive life histories in the North American darters.