The genus Protozetes (Oribatida, Microzetidae) is recorded in Guatemala for the first time. A new species—Protozetes janosbaloghi sp. nov.—is described from damp litter in an alpine cloud forest in Guatemala. It differs from all known species of the genus in the number of the interlamellar apophyses, the morphology of the head of the bothridial seta, the ornamentation of the pteromorph, and the length of the interlamellar and notogastral setae. A revised generic diagnosis is presented. An identification key and data on distribution and habitat of the known species of Protozetes are provided.