Eustigmaeus is one of the largest genera of the Stigmaeidae and contains more than eighty species. Two new species, Eustigmaeus erciyesiensis, ex moss, from Erciyes Mountain (2230m) and Eustigmaeus sculptus, ex soil and litter under different plants, are described from Erzurum, Elazig, Bingöl, Kirikkale and Gümüshane, Turkey. Another species, E. ioanninensis Kapaxidi & Papadoulis, originally found in Greece, is recorded for the Turkish fauna and re-described. A key to the species known from Turkey is provided.