The Draw-a-Scientist Test (DAST) has been extensively researched as a projective test used to assess individuals' perceptions of scientists. This study investigated student perceptions of entomologists and compared responses of students taught by a male instructor with responses of students who viewed video lectures recorded by the male instructor but interacted with a female instructor. Data were collected from two sections of an introductory entomology course at Oklahoma State University, with one section taught by a male instructor and the other by a female instructor using lecture recordings of the male instructor. Drawings were analyzed for characteristics including facial expression, clothing, equipment, presence of insects, race, and gender. The majority of drawings included smiling faces, glasses, and entomology equipment, while few students illustrated persons of color. Specific criteria to classify drawn entomologists as male, female, or non-gender figures were developed and used to assess drawings. The majority of students in both sections drew male entomologists, consistent with previous DAST studies where male scientists predominate. However, a higher proportion of female entomologists were depicted in the section who interacted with the female instructor. These findings emphasize the impact of instructor gender on students' perceptions and stereotypes. Even when lectures are given by a male instructor, interaction with a female instructor can positively influence the gender representation in students' drawings. Efforts should be made to promote diversity and inclusivity in instruction to increase underrepresented groups in science. This study contributes criteria to assess student depictions of gender and provides valuable insights into the gender representation and characteristics depicted in student drawings of entomologists. It highlights the influence of instructor gender on students' perceptions and stereotypes in entomology and likely applies to other fields of biology.