Shoots of Aloina bifrons were regenerated from four herbarium specimens collected from theAmerican southwest, ranging in age from 4 to 12 years old, subcultured to remove field effects, and grown in Petri dishes on native sand for 238 days (70–90 days after gametangial maturation). Rhizautoicy was demonstrated in three of the four genotypes. Plants were protandrous in gametangial development and in time to maturation, although overlap occurred where both sexes were producing mature gametes. Roughly twice as many perigonia were produced than perichaetia. Female shoots were larger than male shoots, yet devoted much less than male shoots to prefertilization reproductive investment. Adjusted to culture area, investment into reproduction on a prefertilization basis was about ten times greater for male function than for female function. Self-fertilization within a single clonal line was accomplished, but sporophytes did not develop beyond the embryonic phenophase. The clonal lines used appear to be self-incompatible, although it is possible that the sporophyte requires a resting phase, colder temperatures, or desiccation in order to complete development.