Italian ryegrass [Lolium perenne L. ssp. multiflorum (Lam.) Husnot] has become a major annual weed in wheat (Triticum aestivum L.) production systems in the inland Pacific Northwest. With large genetic variability and abundant seed production, L. perenne ssp. multiflorum has developed globally 74 documented cases of herbicide resistance covering 8 different mechanisms of action. Harvest weed seed control (HWSC) systems were introduced in Australia in response to the widespread evolution of multiple herbicide resistance in rigid ryegrass (Lolium rigidum Gaudin) and wild radish (Raphanus raphanistrum L.). The efficacy of these systems for any given weed species is directly related to the proportion of total seed retained by that species at harvest time. From 2017 to 2020, ten L. perenne ssp. multiflorum plants were collected from three different slope aspects at each location in Washington, USA. Collections were initiated in each field when it was visually apparent that seed fill was nearly complete, and seed shatter had not yet occurred. Collection continued at near-weekly intervals until the fields were harvested. The number of filled florets on a spikelet was used to assess the degree of seed shatter over time. Seed shatter at harvest was 67% of the total number of florets on each spikelet. Seed shatter was closely aligned with wheat kernel development in both spring and winter wheat. The high percentage of L. perenne ssp. multiflorum seeds that are shattered by harvest may make HWSC less effective than for L. rigidum in Australia; however, seeds with the greatest biomass tend to not shatter before harvest, which may increase the efficacy of HWSC for managing the soil seedbank. Strategies like planting earlier-maturing wheat cultivars could help HWSC be more effective by having wheat harvest begin earlier, when more L. perenne ssp. multiflorum seeds are still on the mother plant.