We describe species richness and habitat associations of mayflies (Ephemeroptera) collected during amphibian surveys of 355 water bodies in Glacier National Park (NP), Montana, in 2006–2008. We collected 9 taxa (in 7 genera) of mayflies that were identifiable to species. Callibaetis jerrugineus hageni was collected most frequently, followed by Siphlonurus occidentalis, S. phyllis, Ameletus celer, A. similior, Parameletus columbiae, Ephemerella dorothea infrequens, Baetis bicaudatus, and Leptophlebia cupida. Siphlonurus phyllis had not been reported in the western United States prior to our surveys, and P. columbiae is a species of concern in the region. The identifications of 4 additional taxa were uncertain due to the poor condition of specimens found at only one site (Centroptilum sp. and Paraleptophlebia sp.) or because nymphal specimens could not be confidently identified (Cinygma sp. and Cinygmula sp.). Species richness of mayflies in wetlands seems low compared to that in streams and lakes in Glacier National Park. We found the most species of mayflies in beaver ponds, where we detected some species not commonly associated with lentic water bodies. Our survey was the first extensive survey of wetland invertebrates in Glacier NP and only the second that we are aware of in western Montana.