We report the first radiocarbon ages on vertebrate fossils from Labor-of-Love Cave, White Pine County, Nevada, based on purified collagen in teeth and bone, as well as a description of the cave's vertebrate paleontology. This cave was discovered in 1982 with the recovery of an associated partial skeleton of the extinct giant short-faced bear (Arctodus simus). Subsequent excavations in 1985 recovered additional fossil material of birds and mammals from stream deposits in the cave. These fossils, along with fossils collected from the surface during surveys in 2018, are reported here. Radiocarbon ages indicate that most fossil material was deposited in the cave before and during the Last Glacial Maximum (LGM; 21,441–27,774 cal yr BP) and eroded from stream deposits inside the cave following the LGM, presumably from increased precipitation and stream flow. The vertebrate assemblage includes 4 other extinct taxa including 1 carnivore (Panthera atrox) and 3 ungulates (Equus sp., Oreamnos harringtoni, Euceratherium collinum) and the first record of Canada lynx (Lynx canadensis) from the Great Basin. If contemporaneous, the assemblage as a whole indicates an open grassland/sagebrush/tundra environment in Spring Valley during the late Pleistocene, with coniferous forest on mountain slopes facing this valley and where the cave is situated. Although the entrance to the cave is now blocked by slumped talus and breakdown, in the Pleistocene it was likely a large accessible opening at the base of a limestone cliff, with stream flow emerging and flowing into the valley below during the late Pleistocene, when bears and other species possibly used the cave as a shelter or den.