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1 October 2012 Molecular Cytogenetic Identification and Characterization ofRobertsonian Chromosomes in the Large JapaneseField Mouse (Apodemus speciosus) Using FISH
Manabu Yamagishi, Kazumi Matsubara, Mitsuru Sakaizumi
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Abstract

Robertsonian (Rb) karyotypic polymorphism in Apodemus speciosus has interested many researchers with particular referece to the genetic divergence between Rb and non-Rb populations. Failure to find morphologic, biochemical, or genetic differences in previous studies reveals the necessity of focusing on loci on Rb chromosomes, which can be characterized by FISH mapping with DNA probes. In an Rb heterozygote, DNA probes from laboratory mouse chromosomes (MMUs) 1 and 10 were simultaneously hybridized to the long arm of a metacentric and a medium-sized acrocentric chromosome and to the short arm of the metacentric and a small acrocentric chromosome, respectively. Four additional probes derived from each of MMUs 1 and 10 were mapped to the long and short arms, respectively, of the Rb chromosome identified by the above markers. Homologies between the long arm of the Rb chromosome and MMU 1 and between the short arm and MMU 10 were supported by all ten markers, which were dispersed along nearly the entire lengths of the Rb chromosomes. These results indicate that the long and short arms of the Rb chromosomes are homologous to Apodemus speciosus chromosomes 12 and 19 (defined in a previous study), respectively. This ten-marker series can be useful for detecting chromosome-specific divergence between the two karyotypic populations at the gene level.

© 2012 Zoological Society of Japan
Manabu Yamagishi, Kazumi Matsubara, and Mitsuru Sakaizumi "Molecular Cytogenetic Identification and Characterization ofRobertsonian Chromosomes in the Large JapaneseField Mouse (Apodemus speciosus) Using FISH," Zoological Science 29(10), 709-713, (1 October 2012). https://doi.org/10.2108/zsj.29.709
Received: 14 December 2011; Accepted: 18 May 2012; Published: 1 October 2012
KEYWORDS
Apodemus speciosus
Fish
genetic marker
non-model animal
Robertsonian translocation
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