Approximately 6000 species of Thysanoptera have been described worldwide. They are particularly diverse in the Neotropics, living in a wide range of environments. Although many species of thrips are well known as inhabitants of cultivated plants, there are others that are abundant in other types of habitats. In the present study we conducted a survey in the “Biosphere Reserve of Volcan Tacana” with the main objective to know the native fauna of thrips. The results confirm the presence of 112 species in 52 genera and 4 families. Our survey indicates that this reserve contains a great biodiversity of Thysanoptera. A full check-list of species is provided.