We present measurements of reflectance spectra from human skin in vivo in the spectral range from 250 to 700 nm. These measurements show that the reflectance from strongly pigmented skin is higher than that from weakly pigmented skin at wavelengths shorter than approximately 300 nm. We simulate the measured results using a new radiative transfer model developed to study light propagation in skin tissue. Our simulations mimic the measured spectra when scattering from melanosomes, and fragmented melanosomes are taken into account. Scattering from microstructures with high relative refractive indices plays a major role in tissue optics. Our results show that scattering from melanosomes and fragmented melanosomes is of particular significance.
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Vol. 80 • No. 3