This study aimed to compare the patterns of postnatal transformation of myosin heavy chain (MyHC) isoform types in the longissimus dorsi (LD) muscle between HeBao (HB) and Large White (LW) pigs, and assess the association of porcine cofilin2b (CFL2b) mRNA abundance with changes of myofiber type composition. The four MyHC isoforms (MyHC-1, -2a, -2b, and -2x) of the LD muscle were assessed for mRNA levels in 28 HB and 28 LW pigs by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The associations of CFL2b mRNA expression and myofiber type (MyHC-1, -2a, -2b, and -2x) changes were analyzed by RT-qPCR. Although the mRNA expression patterns of MyHCs were different between the two breeds, they had similar expression levels. During postnatal growth, relative CFL2b abundance was gradually increased, with dramatic changes observed after 90 d between the two breeds (P < 0.01). Further analysis revealed significant positive correlations of CFL2b gene expression with MyHC-1/slow (HB: r = 0.871), MyHC-2b [LW: r = 0.881 (P < 0.01)], and MyHC-2x (HB: r = 0.795, LW: r = 0.814), and a significant negative correlation with MyHC-1/slow [r = ?0.938 (P < 0.01)] in LW. No significant associations of CFL2b expression with MyHC-2a (HB: r = ?0. 195, r = ?0.697) and MyHC-2b (HB: r = 0.493) were found. Our findings suggested that HB pigs had different muscle development mechanisms in the LD muscle compared with LW, and the CFL2b expression difference could affect the levels of myofiber types which could account for meat quality differences. HB pigs possessed less glycolytic, with more oxidative metabolism and better meat quality traits compared with LW pigs at different growth stages.