Sclerotinia blight is a destructive disease of peanut caused by Sclerotinia sclerotiorum (Lib.) de Bary and Sclerotinia minor Jagger. Crop management practices are routinely used to control Sclerotinia blight, however, development of resistant cultivars together with crop management practices may provide a lasting solution to control the disease in peanut fields. In this study, 95 accessions of United States’ peanut mini-core collection were evaluated using detached leaflet and whole plant inoculation methods under greenhouse conditions. The area of detached leaflet infected was scored using a scale from 0 (no disease) to 4 (76%–100% leaflet area infected). Whole plants were evaluated based on disease severity index (DSI) from 0% (no disease) to 100% (entire plants infected). In the detached leaflet inoculation method, accessions PI-268586, PI-268696, PI-356004, PI-372305, and PI-429420 had the lowest average disease score of 2.7. In the whole plant inoculation method, accessions PI-200441, PI-259658, PI-319770, PI-323268, and PI-337293 had the lowest DSI from 86% to 90%. The two inoculation methods resulted in different set of accessions with the lowest disease level. These results may reflect differences in disease pressure between the two screening methods.