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Five species of lichens are described as new from the Serra de Maracaju in Mato Grosso do Sul (Brazil): Cladonia gumboskii Aptroot, M.F. Souza & Spielmann, sp. nov. (also reported from five other states in Brazil), C. zebrathallina Aptroot & Spielmann, sp. nov., Lecanora fluoroxylina Aptroot & M.F.Souza, sp. nov. (also reported from Mato Grosso and Paraná), L. lichexanthoxylina Aptroot & M.F. Souza, sp. nov., and Trypethelium muriforme Aptroot & M.F.Souza, sp. nov.. A further 123 species are reported new to the area, of which ten are first records for Brazil and a further 41 are first records for the state.