Tardigrades represent one of the most abundant groups of Antarctic metazoans in terms of abundance and diversity, thanks to their ability to withstand desiccation and freezing; however, their biodiversity is underestimated. Antarctic tardigrades from Dronning Maud Land and Victoria Land were analysed from a morphological point of view with light microscopy and scanning electron microscopy, and from a molecular point of view using two genes (18S, 28S) analysed in Bayesian inference and maximum-likelihood frameworks. In addition, indel-coding datasets were used for the first time to infer tardigrade phylogenies. We also compared Antarctic specimens with those from Italy and Greenland. A combined morphological and molecular analysis led to the identification of two new evolutionary lineages, for which we here erect the new genera Acanthechiniscus, gen. nov. (Echiniscidae, Echiniscoidea) and Mesobiotus, gen. nov. (Macrobiotidae, Macrobiotoidea). Moreover, two species new to science were discovered: Pseudechiniscus titianae, sp. nov. (Echiniscidae : Echiniscoidea) and Mesobiotus hilariae, sp. nov. (Macrobiotidae : Macrobiotoidea). This study highlights the high tardigrade diversity in Antarctica and the importance of an integrated approach in faunal and taxonomic studies.

 http://zoobank.org/urn:lsid:zoobank.org:pub:8AAB42BF-B781-4418-A385-DC80C18EC31D

Phylogenetic diversity of Rhagada land snails is high on the Burrup Peninsula, Western Australia, with four distinct clades, representing three of the four major clades of the Pilbara region. Detailed sampling indicated little geographic overlap of the four clades, conforming to the general rarity of congeneric sympatry in Australian camaenids. The diversity on the Burrup Peninsula includes three previously unclassified morphotypes. One of these lies within the broad endemic clade of the adjacent Dampier Archipelago, and is provisionally assigned to the island species R. perprima, based on phylogenetic evidence. The two other undescribed morphotypes constitute an endemic clade that is the sister group of the broader Dampier Archipelago clade. All COI p-distances within clades are less than 6%, whereas nearly all distances between clades exceed 10%, the gap corresponding to differences among species of Rhagada generally. One morphotype in the Burrup Peninsula endemic clade has a low spire and a distinctive keel, and is restricted to a single rockpile. Detailed local sampling revealed gradation between this form and the more widely distributed globose morphotype. On the basis of genetic similarity and morphological continuity, we describe the morphologically variable endemic Burrup Peninsula clade as Rhagada ngurrana, sp. nov., which has a distribution spanning only 9 km.

Insects have developed incredible means to avoid detection by predators. At least five insect orders have species that resemble leaves. Katydids (Orthoptera : Tettigoniidae) are the most diverse and wide-ranging of the leaf-like insects. At least 14 of the 20 extant katydid subfamilies contain species with leaf-like wings. Although it is undisputed that many katydids resemble leaves, methods for delineating the leaf-like from non-leaf-like forms have varied by author and in many cases are not explicitly stated. We provide a simple ratio method that can be used to differentiate the leaf-like and non-leaf-like forms. Geometric morphometrics were used to validate the ratio method. Leaf-like wings have been independently derived in at least 15 katydid lineages. Relative rates of speciation were found to be greater in the non-leaf-like forms, suggesting that leaf-like wings within Tettigoniidae are not a driver of diversification. Likewise, throughout Tettigoniidae, selection seems to be favouring the transition away from leaf-like wings. However, within the large Phaneropterinae subclade, relative speciation and transition rates between the leaf-like and non-leaf-like forms do not differ significantly.

The tropical Asian crab spider genus Pagida Simon, 1895 has remained taxonomically unrevised and has never been subjected to phylogenetic evaluation. The present study is designed to investigate the monophyly of the genus, its placement within Thomisidae and review all species. Our cladistic analysis, based on 78 morphological characters from 34 taxa (30 ingroup and four outgroup), demonstrates the monophyly of the genus and shows that Pagida is sister to Stiphropus Gerstäcker, 1873 within Thomisidae. The monophyly of Pagida and Pagida   Stiphropus is well supported. Pagida salticiformis (O. P.-Cambridge, 1883), the type species of Pagida, is redescribed from a series of specimens collected recently from its type locality, Sri Lanka. Pagida pseudorchestes is redescribed based on nine males and seven females. A new species, Pagida minuta, sp. nov., is described, based on four males and one female.

The land snail Basedowena Iredale, 1937 currently comprises eight species that inhabit mountain ranges in the central Australian arid zone. By studying the variation in shell and genital anatomy and the differentiation in the mitochondrial markers 16S and COI, we revise the taxonomy of this camaenid and describe new taxa. We demonstrate that the current morphology-based classification has been misled by homoplasy mainly in shell shape. In fact, Basedowena as herein revised contains species with vastly different shells, which have so far been assigned to different genera. By contrast, these species have the same general genital anatomy in common and form a clade in the mitochondrial phylogeny. Based on these findings, we transfer Pleuroxia bethana, P. hinsbyi, P. elfina, P. oligopleura, P. polypleura, P. radiata, Montanomelon angatjana and Minimelon colmani to Basedowena. While Pleuroxia and Montanomelon are maintained as distinct genera, although with fewer species, the monotypic Minimelon becomes a junior synonym of Basedowena. We identify and describe three new Basedowena species (B. bicolor, sp. nov., B. holoserica, sp. nov., B. siparium, sp. nov.). A fourth new species differs substantially from all other arid zone camaenids and is hence placed in a new genus, Xeromelon, gen. nov. The revised delineation of Basedowena renders it now the most widespread camaenid genus in Australia. It is distributed throughout the Australian arid zone from Western Australia, via the Northern Territory and South Australia into New South Wales.

Nematodes are important, largely because they frequently act as parasites and threaten the health of plants, animals and even humans. Here, we describe an interesting free-living nematode from land snails on Luofu Mountain, Guangdong, China. Alloionema luofuensis, sp. nov. is phylogenetically related to slug-parasite A. appendiculatum and the well-known vertebrate parasites Strongyloides spp. based on small subunit (SSU) and the D2-D3 domain of large subunit (LSU) rDNA sequences. The new species possesses an extremely transparent body and is easily maintained using C. elegans culture media, suggesting a possible application prospect of this free-living nematode as a comparative model system for its related parasites. Morphology and anatomy of the gonochoristic A. luofuensis, sp. nov. adult were described and illustrated. The species is characterised by a filiform tail bisexually, ‘rhabditiform’ oesophagus and ‘rhabditid-like’ female anatomy, but its male caudal region is completely different from that of typical rhabditid nematodes, being absent from an enveloping bursa. It is the first marker taxon characterised morphologically as well as molecularly from the family Alloionematidae, a group of nematodes with hyperdiverse molecular genetic variations underlying highly conserved anatomy. Further molecular and genetic studies on A. luofuensis, sp. nov. populations hold promise to provide insight into evolution of the clade consisting of vertebrate parasites of the heterogonic nematode genus Strongyloides. This is because of its unusual high levels of heterozygosity maintained by the conserved rRNA genes of partial SSU and the D2-D3 domain of LSU for the type isolate of this species.

Gastrotricha is a cosmopolitan phylum of aquatic and semi-terrestrial invertebrates that comprises ∼820 described species. To date, freshwater gastrotrichs have not been the subject of faunistic or taxonomic research in the polar regions. In this paper, we present the first species-level description of a freshwater gastrotrich from the Arctic (Svalbard Archipelago). Evidence from morphology, morphometry and molecular analyses reveals that the species represents a new genus in Chaetonotidae: Bifidochaetus arcticus, gen. et sp. nov. Taking into consideration many morphological similarities to Chaetonotus (Primochaetus) veronicae Kånneby, 2013 we propose to include C. (P.) veronicae in the newly established genus under the new combination Bifidochaetus veronicae (Kånneby, 2013), comb. nov. In the phylogenetic analysis based on nuclear 18S rRNA, 28S rRNA and mitochondrial cytochrome c oxidase subunit I sequence data, B. arcticus, gen. et sp. nov. is nested within the family Chaetonotidae, as the sister group to the genus Lepidochaetus Kisielewski, 1991. In this paper we also present new taxonomic characters useful for gastrotrich taxonomy: the pharynx-to-intestine length ratio (I) and the spine bifurcation ratio (B).