With the introduction of an invasive pest ant species, Paratrechina sp. nr. pubens, it has become imperative to develop novel control technologies. There is currently no published research concerning dinotefuran and novaluron against pest ants. Paratrechina sp. nr. pubens workers and brood were exposed to baits containing dinotefuran and novaluron at varied concentrations. Liquid bait amended with dinotefuran was applied in the laboratory against P. sp. nr. pubens. Mean percent mortality of P. sp. nr. pubens was typically higher as the concentration increased at both three (F  =  7.28; df  =  28; P < 0.001) and seven (F  =  7.28; df  =  28; P < 0.001) d post-treatments. Three day observations of the lowest concentration (0.00006%) indicated a significantly lower efficacy than the highest two concentrations. LD₅₀ and LD₉₀ values at three and seven d post treatment showed a poor fit to the model (df  =  1; χ²  =  7.20; P < 0.01, df  =  1; χ²  =  7.09; P < 0.01, respectively). The use of dinotefuran was highly efficacious against P. sp. nr. pubens, and is recommended for further laboratory research and initial field research. Corn grit bait amended with novaluron was applied in the laboratory against P. sp. nr. pubens workers and brood. At four wk results did not reveal significant differences among concentrations with active ingredient and controls (F  =  1.504, df  =  3, 27, P  =  0.239). Results of the study were inconclusive regarding the efficacy of novaluron against P. sp. nr. pubens. The findings of this study emphasize the difficulties in maintaining incomplete colonies of P. sp. nr. pubens that contain brood under laboratory settings.