Karine Brenda Barros-Cordeiro, Sônia Nair Báo, José Roberto Pujol-Luz
Journal of Insect Science 14 (83), 1-10, (1 July 2014) https://doi.org/10.1673/031.014.83
KEYWORDS: forensic entomology, metamorphosis, morphology, Stratiomyoidea
The intra-puparial development of the black soldier-fly, Hermetia illucens (L.) (Diptera: Stratiomyidae), was studied based on 125 pupae under controlled conditions in laboratory. The 6th instar larvae were reared until they stopped feeding, and the pre-pupae were separated according to the reduction in larval length and degree of pigmentation and sclerotization of the cuticle. The pupal stage lasted eight days (192 hours). The process of pupation (larva/pupa apolysis) occurred in the first six hours, extroversion of the head and thoracic appendages took place between the ninth and 21st hours, and the pharate appeared 21 hours after completing pupation. After pupariation, four morphological phases of intra-puparial development were observed and described.