In the Andean portion of northern Patagonia, populations of Ctenomys are found from low-elevation plains to high-elevation meadows and valleys. Despite their prevalence, the taxonomy of these subterranean rodents remains poorly resolved. Using genetic and morphological data obtained from museum specimens and animals collected in the field, we examined the taxonomy of Ctenomys from southwestern Mendoza Province, Argentina. Our analyses suggest the presence of at least five species of Ctenomys within the study area. The highest, innermost portion of the Andes is occupied by C. maulinus. To the east, the mountains and foothills are inhabited by two forms associated with the “mendocinus” and the “magellanicus” lineages, respectively. The former appears to be a local variant of C. emilianus, while the latter is sister to C. pontifex. Although C. pontifex was not encountered during our field sampling, it remains a valid species that likely is restricted to the isolated Valle Hermoso in westernmost Mendoza Province. In addition, we report an undescribed form from Las Leñas Valley that is associated with the “mendocinus” lineage. This complex alpha taxonomic scenario occurs within less than one degree of latitude, thereby highlighting the need to conduct detailed field collections to improve our knowledge of the systematics of Ctenomys.