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1 August 2015 Response of Flour Beetles to Multiple Stressors of Parasitic (Hymenolepis diminuta), Environmental (Diatomaceous Earth), and Host (Reproduction) Origin
Allen W. Shostak, Kala G. Van Buuren, Ranon Cook
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Abstract

Organisms face a multitude of potential stressors, and the way these stressors interact can provide insights into underlying biological processes. This study examined the flour beetle Tribolium confusum and its survival, net fecundity, and surface-seeking behavior in response to combinations of stressors from 3 categories. Infection by the cestode Hymenolepis diminuta provided a stress of parasitic origin. Exposure to diatomaceous earth (DE) provided a stress of environmental origin. Use of virgin and mated beetles evaluated reproduction as a stress of host origin. Single and multiple exposure of beetles to parasite eggs achieved a maximum mean abundance of 21 parasites/beetle and a maximum intensity of 90 parasites in an individual beetle. DE reduced initial parasite establishment, but did not directly affect survival of parasites after their establishment in the host. A rehydration technique was used to recover parasites from dead beetles, enabling this to be the first study to correlate H. diminuta intensity at time of death directly to mortality of T. confusum. A dichotomous intensity–mortality relationship was observed in 8% DE, whereby lightly infected (<20 parasites) hosts were killed by DE in an intensity-independent manner, but more heavily infected hosts were killed in an intensity-dependent manner. Host mating status did not affect host survival, but there were interactions among mating status, parasitism, and DE on net fecundity and surface-seeking behavior. However, these effects were minor compared to the host mortality that occurred when parasite abundance and DE concentration were both high. The aggregated distribution of T. confusum in beetles, the difficulty of achieving high mean abundances, and an apparent need for the stressors to have strong effects individually if they are to have enhanced effects when in combination, suggests that exposure to multiple stressors would seriously impact only a small proportion of the host population.

© American Society of Parasitologists 2015
Allen W. Shostak, Kala G. Van Buuren, and Ranon Cook "Response of Flour Beetles to Multiple Stressors of Parasitic (Hymenolepis diminuta), Environmental (Diatomaceous Earth), and Host (Reproduction) Origin," Journal of Parasitology 101(4), 405-417, (1 August 2015). https://doi.org/10.1645/15-733.1
Received: 19 January 2015; Accepted: 1 April 2015; Published: 1 August 2015
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