Foot problems are a common concern in elephant husbandry. Studies on this topic with sample sizes greater than 100 animals have only been carried out in North America. We investigated foot health of 243 Asian elephants (Elephas maximus) in 69 European institutions. During on-site visits between August 2016 and July 2017, standardized pictures were taken of each elephant's nails and pads. The pictures were analyzed with respect to pathological lesions (i.e. nail cracks, abscesses), care issues (i.e. minor abnormalities, which are easily resolvable with routine foot work), and pad structure. Of all analyzed nails and pads, 35.6% revealed varying degrees of pathological lesions, with minor nail cracks and overgrown cuticles with attachment to the nails being most frequently observed. The most lateral nail (N5) on both front feet demonstrated the highest percentage of pathological lesions, providing support to a separate study showing that the mean peak pressure of an elephant's foot occurs along the most lateral digits; however, this was not observed along the most lateral nail (N5) of the rear feet. Three (of 243) elephants did not show any pathological lesions in their feet. The most common issues requiring foot care were fissures in the nail sole. The structure of the pads was categorized in four grades reflecting the percentage of surface marked by sulci. These four grades occurred at nearly equal frequency. Pearson product moment correlations revealed no significant association between the frequency of care issues and pathological lesions per nail. Despite this finding, it may be prudent to implement husbandry protocols that could alleviate commonly observed pathological and care foot issues in captive Asian elephants. A standardized approach to evaluate elephant foot health will provide a more objective way to monitor responses to management and medical decisions and ultimately contribute to the overall wellbeing of elephants in human care.

Front foot radiographs from 22 giraffe (Giraffa camelopardalis reticulata) at one zoo were analyzed to better understand causes of lameness in this giraffe population. The herd had a history of front hoof overgrowth and intermittent lameness. Radiographic findings included distal interphalangeal joint osteoarthritis (OA), distal phalangeal bone (P3) osteitis, P3 fractures, P3 rotation, and sesamoid bone cysts. OA of the distal interphalangeal joint occurred in at least one front foot of 73% (16/22 giraffe) of the herd, and all giraffe had OA by 7 yr of age. Pedal osteitis was present in at least one front foot in 86% (19/22) of the giraffe, starting in animals as young as 1 yr old. P3 fractures were present in 36% (8/22) of the herd. These fractures were near the site of the deep digital flexor attachment and were diagnosed in giraffe as young as 10 yr old. The presence of severe osteitis was associated with the presence of P3 fractures. This study is unique in that a large herd was trained to participate in voluntary front foot radiographs so multiple causes of foot disease could be diagnosed antemortem and without anesthesia. Although the underlying causes of these lesions are likely multifactorial and currently unknown to us, the high prevalence of foot disease in relatively young animals warrants further investigation across zoos. In this study, OA, osteitis, and P3 fractures were common radiographic findings among giraffe that were limping. Subsequent monitoring and management changes suggest that proactive management of foot health can decrease morbidity and mortality in zoo giraffe.

In order to gain an understanding of the frequency of veterinary intervention during the periparturient period of western lowland gorillas (Gorilla gorilla gorilla) in captivity between 1996 and 2016, a survey was sent to institutions associated with the Association of Zoos and Aquariums' Gorilla Species Survival Plan^®. A total of 193 births were reported during the survey period, from 51/53 institutions. There were six incidences of prolonged labor (longer than 6 hr; 3%), six cases of cesarean section (3%), and three incidences of veterinary intervention that did not involve a cesarean section (2%). Fifty-one gorilla neonates (26%) were assist-reared (required intervention lasting longer than 24 hr). Out of 51 attempts to reintroduce neonates to dams or surrogates, 42 were accepted (82%), while nine attempts (18%) were deemed failures. The age group with the highest rate of maternal or surrogate acceptance after reintroduction was between 6 and 12 mo of age. Compared to data from a 1981 survey, the percentage of assist-reared gorillas decreased from 64% to 26%. Results show that veterinary intervention in the periparturient period is low, and there is a high rate of acceptance of neonates by either the dam or a surrogate after intervention. Advances in captive husbandry and veterinary knowledge have led to a reduced risk of veterinary intervention during gorilla parturition; however, the long-term effects on behavioral development of the neonate should be considered before removal of the infant from the dam for any period of time.

Herpesvirus infections have been associated with high morbidity and mortality in populations of captive emydid chelonians worldwide, but novel herpesviruses have also recently been identified in apparently healthy free-ranging emydid populations. Blanding's turtle (Emydoidea blandingii), an endangered species in Illinois, has experienced range-wide declines because of habitat loss, degradation, and fragmentation. A novel herpesvirus, Emydoidea herpesvirus 1 (EBHV1), was identified in Blanding's turtles in DuPage County, IL, in 2015. Combined oral-cloacal swabs were collected from radio transmitter–fitted and trapped (n = 54) turtles multiple times over the 2016 activity season. In addition, swabs were collected at a single time point from trapped and incidentally captured (n = 84) Blanding's turtles in DuPage (n = 33) and Lake (n = 51) counties over the same field season. Each sample was tested for EBHV1 using quantitative polymerase chain reaction (qPCR). EBHV1 was detected in 15 adult females for an overall prevalence of 10.8% (n = 15/138; 95% confidence interval [CI]: 6.2–17.3%). In radio transmitter–fitted females, there was a significantly higher prevalence of EBHV1 DNA in May (23.8%, n = 10/42) than June (3.6%, n = 1/28), July (0%, n = 0/42), August (0%, n = 0/47), or September (7.7%, n = 3/39) (odds ratio: 12.19; 95% CI: 3.60–41.30). The peak in May corresponds to the onset of nesting and may be associated with increased physiologic demands. Furthermore, all positive turtles were qPCR negative in subsequent months. There were no clinical signs associated with EBHV1 detection. This investigation is the critical first step to characterizing the implications of EBHV1 for Blanding's turtle population health and identifying management changes that may improve sustainability.

Investigation into antemortem semen collection methods in reptiles is required to counteract anthropogenic induced reptile biodiversity loss and to further develop assisted reproductive technologies in reptiles. The purpose of this study was to evaluate whether repeated electroejaculation under anesthesia could be done safely in the veiled chameleon (Chamaeleo calyptratus). Electroejaculation under anesthesia (alfaxalone 15 mg/kg intravenously) was performed once weekly over a 4-wk period in 12 Chamaeleo calyptratus. External vent appearance and cloacoscopy was performed prior to and after electroejaculation to assess the effect of the electroejaculation on the external and internal anatomy. Hematological and chemistry values were evaluated prior to the study and afterwards. Semen collection was successful in 50% of the electroejaculation events. Cloacoscopy images scores were significantly higher between the pre- and postelectroejaculation events for each week. No significant difference was observed between study week 1 prior to electroejaculation and study week 4 after electroejaculation in vent external morphology (P = 0.157), and internal cloacal scoring (P = 0.058). A significant increase in the white blood cell counts (P = 0.015), absolute heterophil counts (P = 0.02), absolute monocyte counts (P = 0.003), and albumin (P = 0.038) was observed between the pre- and postejaculation sampling periods. Aspartate aminotransferase (P = 0.001) and potassium (P = 0.036) decreased significantly between the pre- and postsampling periods. Vent image scores were significantly higher (P = 0.046) between the pre- and postsampling images collected during week 1; however, there were no significant differences noted between pre- and postsample images for weeks 2 (P= 0.063), 3(P= 0.083), or 4 (P= 0.083). Serial electroejaculation procedures under alfaxalone anesthesia can be performed safely in veiled chameleons. Although changes were observed in the complete blood count and biochemistry results, these changes were determined to not be clinically significant. The results of this study demonstrate that antemortem assisted reproductive techniques such as electroejaculation can be safely used repeatedly.

Published anesthetic protocols for captive and free-ranging bears are limited to injectable inductions with maintenance via inhalants or additional injectable boluses. Though common in other species, intravenous (IV) continuous-rate infusions (CRI) using guaifenesin combinations have not been evaluated in ursids. This study evaluated the use of a CRI compared to an inhalant for maintenance anesthesia. Seven healthy American black bears (Ursus americanus) were anesthetized in a crossover design with two different anesthetic maintenance protocols. Bears were immobilized with ketamine (2.02 ± 0.14 mg/kg) and medetomidine (0.04 ± 0.003 mg/kg) for both protocols. The anesthetic maintenance control protocol consisted of isoflurane gas (ISO) started at 2% delivered by endotracheal tube; the experimental protocol consisted of guaifenesin, medetomidine, ketamine (GMK) IV CRI started at 50 mg/kg/hr guaifenesin, 0.01 mg/kg/hr medetomidine, and 1 mg/kg/hr ketamine. Induction and recovery parameters including time to first effect, recumbency, and hands on; duration of maintenance protocol; and time from reversals administered to head up, standing on all four feet, no ataxia, and to fully recovered were recorded and compared between protocols. Heart rate, respiratory rate, rectal temperature, blood pressure, end tidal carbon dioxide, and hemoglobin oxygen saturation were recorded at 5-min intervals and compared between protocols. Venous blood gases were obtained at the start, middle, and end of the maintenance anesthesia and compared between protocols. All bears exhibited hypertension with mild respiratory acidosis throughout procedures. Measured physiologic parameters did not differ significantly between the isoflurane and the GMK CRI maintenance protocols, with the exception of higher endpoint (ISO) pCO2 measurements. No adverse events were recorded with either protocol, and adequate depth of anesthesia was maintained with both protocols. GMK CRI provides a safe, effective, and more portable alternative to inhalant anesthetics for maintenance anesthesia in bears in captivity or in the field.

The study objective was to evaluate the sedative, muscle relaxant, and cardiorespiratory effects of midazolam and flumazenil in the ball python (Python regius). Ten healthy adult female ball pythons were used in a randomized and blinded crossover trial evaluating the effects of two dosages (1 and 2 mg/kg intramuscular [i.m.] in the cranial third of the body). In a subsequent open trial, nine ball pythons received 1 mg/kg i.m. of midazolam followed by 0.08 mg/kg i.m. of flumazenil 60 min later. Heart rate, respiratory rate, temperature, and the level of sedation and muscle relaxation (using a semiobjective scoring system) were evaluated. There were no significant differences between midazolam dosages for any of the parameters evaluated. Sedation scores were significantly increased compared with baseline from 15 min (1 mg/kg) and 10 min (2 mg/kg) postinjection up until 56 hr (1 mg/kg) and 72 hr (2 mg/kg) postinjection. Peak effect was reached 60 min postinjection, with 60% of snakes (6/10) being unable to right themselves. One snake developed paradoxical excitation with the 2 mg/kg dosage. Heart rates were significantly lower than baseline from 30 min to 128 hr postinjection with both midazolam dosages. Respiratory rates were significantly lower than baseline at four time points, with the highest dosage only: 15, 45, 60 min, and 8 hr postinjection. Flumazenil resulted in reversal of sedation and muscle relaxation in all snakes within 10 min of administration. However, resedation was evident in all snakes 3 hr after reversal. Midazolam administered at 1 and 2 mg/kg i.m. provides a moderate to profound, although prolonged, sedation and muscle relaxation in ball pythons. Flumazenil reverses the effects of midazolam in ball pythons, but its duration of action at the evaluated dosage is much shorter than midazolam, leading to resedation.

Numerous conditions, from acute to chronic renal changes, have the potential to alter the renal size on radiographs. However, current literature does not provide any radiographic reference range for the normal renal size in the serval (Leptailurus serval). This retrospective study includes 13 healthy adult servals, seen between 2004 and 2017 at the UTCVM Veterinary Medical Center, deemed healthy based on physical examination and blood work. Orthogonal radiographs were anonymized, calibrated, and evaluated blindly by three observers of different experience levels. Renal and individual lumbar vertebral body lengths were measured on both orthogonal views, and ratios of kidney length to each lumbar vertebral length were calculated on both views. On the ventro-dorsal projection, the kidney length to L5 vertebra ratio had the lowest standard deviation (SD) with a mean of 1.82, SD of 0.27, and 95% confidence interval (CI) (1.63–2.00). On the lateral view, the kidney length to L3 vertebra ratio had the lowest SD with a mean of 2.03, SD of 0.25, and 95% CI(1.86–2.20). Statistically, all three observers were shown to be in agreement, indicating reproducibility of the measurements among observers with different levels of expertise.

Health assessment of free-ranging populations requires an integrated approach, often incorporating a method to measure mass as a representation of the animals' ability to utilize environmental resources. In chelonians, direct measurements of mass have historically served as a corollary for body condition. However, this method may not accurately represent the true fat volume (FV) and may be skewed by the presence of eggs, shell size, or muscle mass. The objective of this study was to use computed tomography (CT) to develop a model for determining body condition index (BCI) in free-ranging Blanding's turtles (Emydoidea blandingii). Mass, shell measurements, and FV were measured by CT in 65 free-ranging Blanding's turtles from Lake and DuPage counties in Illinois. Twenty-one different models were built for BCI using both FV and fat percentage (FP) as dependent variables. The best fit model for FP included the relationship between mass and carapace length with nearly 60% model support. The model for FV demonstrated a similar relationship but had only 18% support. Linear models with BCI as the dependent variable showed that juveniles had a higher FP than adults and females with more eggs had a lower FP. FP can be calculated in the field with nearly 60% accuracy compared to CT-assessed FP as a component of a physical exam and population health survey to assess the effectiveness of conservation efforts for the endangered Blanding's turtle.

Relatively little is known about pangolin care and physiology, and it has become increasingly important to have a better understanding of normal health parameters for evaluation of free-ranging, rehabilitating, and managed populations. Echocardiograms were performed on six African white-bellied pangolins (Phataginus tricuspis) under professional care at a single institution. Because of the propensity of pangolins to roll up with only thick keratinized scales exposed, the pangolins were anesthetized with either isoflurane or sevoflurane. All echocardiograms were performed and evaluated by a board-certified cardiologist. None of the pangolins had any evidence of prior cardiac disease. All standard measurements were obtained. Four pangolins (66.7%) had trace to mild mitral regurgitation, six (100%) had trace to mild tricuspid regurgitation, and two (33.3%) had trace to mild pulmonic regurgitation. All examinations were determined to be normal, with no evidence of valvular structural abnormalities, cardiac dysfunction, chamber enlargement, or cardiac hypertrophy. This report broadens understanding of normal echocardiogram parameters in pangolin species and contributes techniques and baseline data for veterinarians caring for these vulnerable populations.

Electrophoresis is a useful diagnostic tool for the detection of shifts in plasma protein fractions, which can be associated with a variety of diseases. This method is not often used in reptile medicine because there are only a few studies available and no reference intervals have been published for most species. The goal of this study was to establish reference intervals for plasma capillary zone electrophoresis in Hermann's tortoises (Testudo hermanni) under consideration of variations by season and sex. 251 samples from healthy adult Hermann's tortoises were collected from May 2016 to October 2017. Total protein was measured by the biuret method and albumin was measured twice by bromocresol green dye-binding method (BCG) and capillary zone electrophoresis (CZE) in order to compare the detection methods. The globulin fractions were analyzed by CZE. Seasonal variations were significant for total protein (P = 0.0055), albumin (P = 0.0322) and α-globulins (P = 0.0007). The variations between male and female Hermann's tortoises were highly significant for albumin (P < 0.0001), α-globulins (P < 0.0001) and β-globulins (P < 0.0001). The differences between the measurements of albumin by CZE and BCG also differed depending on the sex and season. This study demonstrates that it is important to include the variations by sex and season when establishing reference intervals and interpreting results. Furthermore, it confirms the importance of using electrophoresis for the correct determination of albumin in tortoises.

American flamingos (Phoenicopterus ruber) are commonly kept in zoological collections, making health monitoring essential. Use of point-of-care (POC) blood analyzers that require small volumes of whole blood samples produces prompt results allowing for rapid clinical decision-making. To evaluate and compare blood biochemistry analysis results analyzed by a POC biochemistry analyzer and a laboratory wet biochemistry analyzer, blood was collected from 17 apparently healthy zoo-kept American flamingos. Analyzer agreement was investigated using the Passing–Bablock regression analysis and Spearman correlation coefficients. Plasma samples from all birds were bright yellow in color. The results from the POC analyzer used in this study were found to be outside acceptance and clinical allowable error limits when compared with the laboratory analyzer for phosphorus (Phos), total protein (TP), albumin (Alb), glucose (Glu), creatine kinase (CK), and potassium (K). For aspartate aminotransferase (AST), results were within clinical allowable error but outside the acceptance limits, and for calcium (Ca) and sodium (Na), results were within both limits. The POC analyzer failed to measure the uric acid (UA) concentrations of all the samples, and reported all bile acids (BA) concentrations as below its minimal measurable limit. The use of analyzer-specific reference intervals is recommended for most analytes tested. The POC analyzer used in this study cannot be recommended for measuring UA concentrations in brightly colored samples from American flamingos.

Domestic and nondomestic ruminant neonates with failure of passive transfer of immunity (FPI) have an increased risk of morbidity and mortality. Hand-reared individuals often have a higher incidence of FPI compared with those that are dam-reared. Hand-reared, nondomestic ruminant neonates raised at zoological establishments are often fed a commercially available bovine colostrum replacer. Consequently, it is desirable to monitor for FPI using a single test that performs well across a wide range of species. The immunocrit test method has been reported to have acceptable diagnostic sensitivity for detecting FPI in dairy calves. Advantages of the immunocrit are that it is inexpensive, quick, easy to perform, non-species-specific, and only precipitates immunoglobulins. This study evaluated the ability of immunocrit to predict serum immunoglobulin levels and FPI via comparison with radial immunodiffusion (reference method). The study used 100 serum samples collected from hand-reared ruminant neonates from 29 nondomestic species, 12–120 hr old, that were fed a commercial bovine colostrum replacer at a zoological institution. Sensitivity, specificity, and likelihood ratios for FPI were determined using receiver operating characteristic (ROC) curves. The immunocrit cutoff point of 14% maximized sensitivity at 88.6% (95% CI, 78.7–94.9%) and specificity at 83.3% (95% CI, 65.3–94.4%). The area under the ROC curve was 0.92 (95% CI, 0.87–0.98; P < 0.001). This research demonstrates that the immunocrit test has acceptable performance in detecting FPI in a wide variety of nondomestic hand-reared ruminant neonates. Use of the immunocrit test will help improve the identification of FPI neonates and aid in the management of colostrum feeding practices of nondomestic ruminant species in captivity.

Schirmer tear test (STT), intraocular pressure (IOP), and ocular biometry were measured in 58 healthy cheetahs (Acinonyx jubatus) between the ages of 2 and 14 yr in two captive groups. During routine health examination under immobilization, each animal underwent ophthalmic examination including STT, IOP, slit-lamp biomicroscopy, indirect ophthalmoscopy, and ocular ultrasonography. Mean ± SD values for STT and IOP were 13.58 ± 5.29 mm/min and 31.40 ± 5.01 mm Hg, respectively. The time between immobilization and examination significantly influenced both STT and IOP measurements. For IOP measurements, a significant group effect was also demonstrated, possibly due to the different anesthetic drug combinations used. Mean ± SD values for biometry were as follows: globe length (32.73 ± 1.74 mm), lens thickness (7.25 ± 1.09 mm), anterior chamber (8.54 ± 1.21 mm), and posterior segment (16.31 ± 2.06 mm). Surprisingly, all of the biometry measurements differed significantly between the two groups. Establishing STT, IOP, and biometric reference values is important for evaluating the ocular health status of the species. These reference values will assist veterinarians working with cheetahs to perform a full and informed ophthalmic examination.

Venous blood samples were collected from 64 apparently healthy juvenile Eastern gray squirrels (Sciurus carolinensis) after sedation with midazolam at the Wildlife Center of Texas located in Houston, Texas, during 2012. Blood gas (pH, PCO2, PO2, base excess, bicarbonate, oxygen saturation), electrolyte (sodium, potassium), biochemical (total CO2, ionized calcium, glucose), and hematologic parameters (hematocrit, hemoglobin, complete blood count) were determined using the i-STAT point-of-care analyzer. Sex did not affect any analyte. All squirrels recovered uneventfully and were successfully rehabilitated and released. Most values were as expected based on comparison to other young rodent species. These analyte data for healthy juvenile Eastern gray squirrels may be useful in assessment of Eastern gray squirrel population health and management and treatment of individual squirrels presented in need of medical care.

The brown lemur population (Eulemur fulvus spp.) in Mbouzi islet is not native, and was introduced in 1997. Since then, the population has grown. In 2012 the National Council for Protection of Nature of Mayotte requested to remove this population of lemurs from Mbouzi, as they were suspected to be a threat to the protected endemic flora of the islet. The Association Francophone des Vétérinaires de Parcs Zoologiques (French-speaking Zoo Veterinarians Association, AFVPZ) was asked to conduct a biomedical evaluation of the population. Fifty-two animals were captured, anesthetized, and weighed. They all underwent a general physical examination. Feces were sampled for bacterial and parasitological screening. Hair was sampled for genetic studies and blood was sampled for hematology, biochemistry, viral serology, and haemoparasitology. Results showed that three individuals had a positive feces culture for Salmonella enterica and six had Lemuricola or Callistoura parasite infestations. Blood analyses for hematology and biochemistry showed 46 animals with elevated transferrin, 42 with low ferritin levels, 19 with hyperglycemia, and 10 with neutrophilia. Finally, 10 were positive for Toxoplasma serology, one was positive for α herpesvirus, five for pox virus, five for simian virus 40, and two for flavivirus. This publication reports the first complete biomedical evaluation of lemurs on Mayotte Island.

The aim of this research was to determine what veterinary students in Australia and New Zealand consider important for animal welfare and ethics (AWE) competence when dealing with wildlife, and to determine how these priorities correlate with gender and stage of study. These students were asked to state their gender and stage of veterinary education and to rank the importance of six AWE topics: (1) “disaster preparedness,” (2) “veterinarians' duties to wild animals,” (3) “methods and justification for wild animal use” (e.g., harvesting/ hunting, wildlife parks), (4) “tensions between animal-welfare concerns and environmental concerns,” (5) “the nature and status of semiowned animals,” and (6) “euthanasia,” pertaining to wildlife for competence on the first day after their graduation. Data were then analyzed. Of 3,320 students invited to participate, 556 responded to questions about animals in the wild. The AWE topic ranked as the most important was “veterinarians' duties to wild animals,” followed by “euthanasia.” Senior students ranked “euthanasia” as the most important topic. The rankings of “methods and justification for wild animal use” and “tension between animal welfare and environmental concerns” were significantly less important for students in the later years of study than for those in early years. Male respondents ranked “euthanasia” as more important than female respondents did, especially in later years of study. Senior veterinary students ranked “euthanasia” as the most important AWE topic for day one competency.

Blood pressure assessment is valuable during management of chronic conditions with increased risk of developing hypertension and as a standard practice for anesthetic monitoring. Normal arterial blood pressure values have not been well described in megachiropteran species. Following anesthetic induction and maintenance with isoflurane in oxygen, arterial blood pressure was obtained from the posterior tibial artery of eight large flying foxes (Pteropus vampyrus) and six variable flying foxes (Pteropus hypomelanus), two with structural cardiac disease and four in good clinically health. Normal values reported as a median with interquartile range for systolic, diastolic, and mean (MAP) arterial pressures for P. vampyrus were 101 (94, 107), 69 (57, 80), and 86 (75, 93), respectively. Normal MAP for clinically healthy P. hypomelanus was 86 (67, 93). Placement of P. hypomelanus in a vertical head-down position did not alter blood pressure in clinically healthy bats, but significantly increased MAP in two bats with structural cardiac disease. Arterial catheterization of both the posterior tibial and median arteries in these species was easily performed without major complication.

Ophidiomycosis, historically referred to as snake fungal disease (SFD), caused by Ophidiomyces ophiodiicola, is a significant disease of snakes characterized by crusty scales, pustules, subcutaneous nodules, and death. Ophidiomycosis is a proposed threat to sustainability of free-ranging snake populations throughout the United States and Europe, but the clinical progression during periods of reproductive activity (gravid females, neonates) is unknown. In spring 2012, five apparently healthy gravid eastern massasauga (Sistrurus catenatus) rattlesnakes from Clinton County, Illinois, were brought into captivity to give birth and be returned into the population. While in captivity, one adult female and 21 neonates died. Five individuals were subsequently confirmed positive for O. ophiodiicola by using quantitative polymerase chain reaction (qPCR). In 2016, a gravid timber rattlesnake (Crotalus horridus) with ophidiomycosis from Jackson County, Illinois, gave birth in captivity to 13 neonates. Skin swabs were taken from all neonates immediately after birth and confirmed negative for O. ophiodiicola by using qPCR. The neonates remained housed with the positive female for 10 days before all animals were reswabbed and released back into the wild. One neonate was O. ophiodiicola positive at time of release. The initial negative result followed by a positive result several days postpartum suggests that the neonate was infected by the female after direct contact. Both case series represent natural infection of neonates after parturition and highlight the importance of this disease in a demographically important age class.

There are no studies to date on the normal reproductive physiology of African white-bellied pangolins (Phataginus tricuspis). As a reclusive species, little is known about normal gestation, successful parturition, and potential complications during pregnancy. Ten female P. tricuspis were diagnosed as pregnant and monitored under professional care. Five developed complications during pregnancies or during parturition and are detailed in this case series. Dystocia occurred in two dams each, with malposition of a singleton fetus. Both dams were successfully treated with surgical intervention by caesarian section. Of the two individuals, one fetus was nonviable, but the other neonate survived and was reared by the dam to weaning. A third pregnant female died during pregnancy from septicemia resulting in death of the preterm fetus. The two additional dams of the five gave birth to full-term neonates. One fetus was stillborn with evidence of fetal distress, and the other died immediately after birth (perinatal death) with undetermined etiology. Based on this case series, complications associated with pregnancy occur in P. tricuspis, indicating the need for further study and close monitoring during impending parturition.

Three sibling snow leopard (Panthera uncia) cubs were evaluated shortly after birth and found to have bilateral eyelid colobomas involving >50% of the upper central and lateral lid margins. The cubs also had iris to cornea persistent pupillary membranes and developed varying degrees of keratitis caused by trichiasis and exposure. No fundic abnormalities were noted. Given the severity of the defects an extensive surgical correction was required. Prior to surgical correction, the cubs were separated from the dam daily and a human-animal socialization plan was enacted to prepare the cubs for the intensive postoperative care that would be required. Bilateral lip commissure to eyelid transposition surgeries were performed on one cub at a time at 2 wk intervals starting at 3 mo of age. Postoperative care was labor and time intensive and each cub was maintained at the zoo's veterinary hospital for 11–21 days with daily supervised visits with the cub's siblings. All cubs were successfully reintroduced together and with the dam at the end of each hospitalization. The surgical procedure yielded fully functional eyelids and an excellent cosmetic appearance in all three cubs. The planned consistent human interaction with the cubs before and during the postoperative period made the procedure and its intensive aftercare possible and was crucial to the successful outcome of the surgeries.

A group of five juvenile Meller's chameleons (Trioceros melleri) experienced 100% mortality over a period of 1 mo due to ranavirus infection. The index case was found dead without premonitory signs. The three subsequent cases presented with nonspecific clinical signs (lethargy, decreased appetite, ocular discharge) and were ultimately euthanatized. The final case died after initially presenting with skin lesions. Postmortem examination revealed thin body condition in all five animals and mild coelomic effusion and petechiae affecting the tongue and kidneys of one animal. Microscopically, all animals had multifocal necrosis of the spleen, liver, and kidney; four of five animals had necrosis of the nasal cavity; and two of five had necrosis of adrenal tissue, bone marrow, and skin. Numerous basophilic intracytoplasmic inclusions were present in the liver of all animals and nasal mucosa of three of the five animals. Consensus polymerase chain reaction for herpesvirus and adenovirus were negative, whereas ranavirus quantitative polymerase chain reaction was positive. Virus isolation followed by whole genome sequencing and Bayesian phylogenetic analysis classified the isolates as a strain of frog virus 3 (FV3) most closely related to an FV3 isolate responsible for a previous outbreak in the zoo's eastern box turtle (Terrapene carolina carolina) group. This case series documents the first known occurrence of ranavirus-associated disease in chameleons and demonstrates the potential for interspecies transmission between chelonian and squamate reptiles.

An outbreak of brucellosis occurred in a group of 726 sand gazelles (Gazella marica) at the Prince Mohammed Al-Sudairi Gazelle Breeding Center and in a group of 47 putative “Neumann's gazelles” (Gazella erlangeri) housed at the King Khalid Wildlife Research Center in Saudi Arabia. Clinical signs of anorexia, poor body condition, enlarged testes, reluctance to walk, swollen carpal joints, and suppurative arthritis were present in 16 sand gazelles and 14 Neumann's gazelles. All clinical cases were evaluated using a card agglutination test, complement fixation, and enzyme-linked immunosorbent assay. All cases were serologically positive for Brucella melitensis, confirmed through culture and isolation of the microorganism. DNA was extracted from the isolated organisms, and polymerase chain reaction (PCR) was used for sequencing. Evaluation focused on tracking the source of infection, the management of the two outbreaks, and the subsequent diagnosis, treatments, and success, including the successful eradication of Brucella from both populations.

An onset of respiratory disease in a captive bachelor group (n = 3) of western lowland gorillas (Gorilla gorilla gorilla) was concomitant with peak attendance of visitors at the institution and with unwanted occurrences of food items being thrown in the gorillas' enclosure. While the condition of two individuals improved with supportive therapy and antibiotics, the third gorilla died three days following initiation of treatment. A fatal bacterial pneumonia, secondary to an infection by a human parainfluenza virus 2 (HIPV-2), was considered to be the cause of death based on histopathology, lung cultures, and reverse transcription PCR. HPIV-2 activity in the human population of the province was detected for that period, including the same viral strain. This report confirms a HPIV-2 respiratory illness and associated death in a gorilla. Clinical presentation and context suggest conspecifics were also affected and that contaminated food thrown by visitors may have been the source of infection.

The present study evaluated the effects of fluoxetine on decreasing stereotypic behaviors in Asiatic black bears (ABB). Four captive ABB exhibiting stereotypic behaviors were administered fluoxetine (0.25–1 mg/ kg orally every 24 hr) for 91 days. The animals were monitored twice weekly for 213 days (April to October) using a scan sampling method (preadministration, 30 day; administration, 91 days; postadministration, 92 days). Five blood samples were collected from the jugular vein of each bear for serum chemistry analysis (one during preadministration; two each during administration and postadministration periods). Fluoxetine was safe and effective in decreasing stereotypies, but it may be difficult to decrease long-established stereotypies in ABB using fluoxetine alone. To decrease stereotypies in ABB effectively, it is necessary to identify underlying problems that contribute to stereotypic behavior and apply comprehensive interventions that can include fluoxetine treatment.

Parelaphostrongylus andersoni, the muscleworm, commonly infects white-tailed deer (Odocoileus virginianus) and also infects caribou (Rangifer tarandus [R.t.] groenlanicus, R.t. grantii, R.t. tarandus, and R.t. caribou). Heavy infection with P. andersoni leads to weakness in the hindquarters, abnormal gait, and pulmonary lesions. The geographical range and full host spectrum of this parasite are not fully known. This study aims to understand host specificity better, especially in nonnative cervids and bovids. This study involved the collection of 140 fecal samples from native and nonnative cervid and bovid species, and 34 snail specimens. With the use of real-time PCR, we found 4/47 (8.5%) O. virginianus fecal samples were positive for P. andersoni. No previously undocumented species of cervids or bovids were found to be infected. Further research is warranted to understand P. andersoni range, host distribution, and potential impact on host health.

A captive 8-yr-old female mountain coati, Nasuella olivacea, presented with intermittent paresis of the pelvic limbs. The coati was anesthetized for radiographs, which showed mineralized discs in the thoracolumbar region. After 3 mo of daily prednisone, its condition further declined. Magnetic resonance imaging confirmed extradural disc herniation, spinal cord compression, and requirement for a left-sided hemilaminectomy. Postoperatively, the coati received prednisolone, gabapentin, tramadol, and clavamox. An established history of positive reinforcement training allowed caretakers to implement physical therapy walks and novel exercises designed for its condition. The coati showed improvement with appropriate proprioceptive positioning and improved balance. Intervertebral disc disease is common in dogs and cats, and although physical therapy is routinely implemented in recovering neurologic patients of those species, it is relatively new in zoologic medicine. This report highlights the benefits of behavioral management in postoperative management of nondomestic species.

Juvenile whooping cranes (Grus americana) raised for wild release were found to have an increased incidence of rib fractures at fledging in 2017 compared with the previous 16 years. Serum analysis showed 30-day-old juveniles in 2017 (n = 12) had significantly lower 25-hydroxyvitamin D3 and significantly higher parathyroid hormone concentrations than juveniles in 2010 (n = 6) with no history of rib fractures. Increased serum parathyroid hormone concentrations in the 2017 juveniles persisted to fledging age. Review of dietary and environmental management revealed that juveniles in 2017 were provided a commercial diet with a lower, and perhaps suboptimal, calcium:phosphorus ratio and experienced reduced time outdoors in the first month after hatch, presumably resulting in less ultraviolet B radiation exposure. Mild hyperparathyroidism in precocial whooping cranes may result when dietary constraints and/or outdoor access is compromised and manifest as rib fractures in the absence of traumatic injury.

The synthesis and circulating concentrations of acute phase proteins (APPs) are regulated in response to inflammation, infection, trauma, and neoplasia in many domestic and nondomestic species. The APP response is species specific; thus, assays must be validated, and reference intervals must be determined for each species. Koalas (Phascolarctos cinereus) are a vulnerable species, threatened by infectious and inflammatory diseases both under human care and in the wild. The ability to diagnose, treat, and provide prognosis for common koala health problems is challenged by the paucity of sensitive diagnostic tests. Assays for C-reactive protein, serum amyloid A, and haptoglobin were validated for use in koalas. Reference intervals were established using the robust method recommended by the American Society for Veterinary Clinical Pathology based on serum samples from 26 healthy koalas at the San Diego Zoo. The reference intervals are as follows: C-reactive protein, 3.2–24.1 mg/L; serum amyloid A, 0.10–0.45 mg/L; haptoglobin, 0.10–0.64 mg/ml.

Five cerebrospinal fluid (CSF) samples were collected from four clinically normal grizzly bears from the Washington State University Bear Research, Education, and Conservation Center. CSF samples were collected from the cerebellomedullary cisternal space. Samples were immediately processed and analyzed for microprotein content, red blood cells, white blood cells (WBCs), and differential cell count. Microprotein concentration (range 4.2–14.6 mg/dl; median, less than 6 mg/dl), total WBC count (range 0–2 cells/µl; median 2 cells/µl), and differential WBCs (predominance mononuclear cells) of the five CSF samples were comparable to previously published CSF analyses from clinically normal felines and canines. Providing documentation of CSF composition for clinically normal grizzly bears is important for neurologic disease diagnosis and treatment.

The population of Tachypleus tridentatus, a horseshoe crab, has decreased sharply in Japan. With the exception of a few reports regarding the morphological features of T. tridentatus, a three-dimensional analysis of its inner anatomical structures has never been documented. Here, the anatomical structures in two T. tridentatus specimens were examined using magnetic resonance imaging (MRI). In the larger of the two specimens, the three-dimensional T2-weighted image clearly revealed muscles, digestive and urinary systems, ovary, and brain; however, the cardiovascular system was poorly visualized. In the smaller specimen, cardiovascular, digestive, and urinary systems were identifiable. In the three-dimensional T1-weighted images, tissue contrast was low, making it challenging to distinguish the individual organs. These results suggest that MRI is a powerful tool for visualizing the inner anatomical structures of horseshoe crabs.