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1 August 2001 Probing the Spatial Dependence of the Emission Spectrum of Single Human Retinal Lipofuscin Granules Using Near-field Scanning Optical Microscopy
Nicole M. Haralampus-Grynaviski, Laura E. Lamb, John D. Simon, Jeffrey R. Krogmeier, Robert C. Dunn, Anna Pawlak, Malgorzata Rózanowska, Tadeusz Sarna, Janice M. Burke
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Abstract

The emission spectra of single lipofuscin granules are examined using spectrally resolved confocal microscopy and near-field scanning optical microscopy (NSOM). The emission spectrum varies among the granules examined revealing that individual granules are characterized by different distributions of fluorophores. The range of spectra observed is consistent with in vivo spectra of human retinal pigment epithelium cells. NSOM measurements reveal that the shape of the spectrum does not vary with position within the emissive regions of single lipofuscin granules. These results suggest that the relative distribution of fluorophores within the emissive regions of an individual granule is homogeneous on the spatial scale ∼150 nm.

Nicole M. Haralampus-Grynaviski, Laura E. Lamb, John D. Simon, Jeffrey R. Krogmeier, Robert C. Dunn, Anna Pawlak, Malgorzata Rózanowska, Tadeusz Sarna, and Janice M. Burke "Probing the Spatial Dependence of the Emission Spectrum of Single Human Retinal Lipofuscin Granules Using Near-field Scanning Optical Microscopy," Photochemistry and Photobiology 74(2), 364-368, (1 August 2001). https://doi.org/10.1562/0031-8655(2001)074<0364:PTSDOT>2.0.CO;2
Received: 22 February 2001; Accepted: 1 April 2001; Published: 1 August 2001
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