Nycteridopsylla iae Beaucournu and Kock, 1992 and Nycteridopsylla quadrispina Lu and Wu, 2003 have heretofore been known only by the male and female sexes, respectively. The morphological differences in these two species are striking; however, a recent collection of male N. iae and female N. quadrispina from a single host individual of the Great Evening Bat (I. io Thomas), led us to suspect that N. iae and N. quadrispina represented the same species. Morphological similarities were noted and male and female exemplars were sequenced using five genes (18S, 28S, 16S, 12S, and COII). Across the five sequenced genes from both specimens (male and female), sequence identity was 100%. Considering morphological characteristics and DNA analysis, N. quadrispina is indisputably a junior synonym of N. iae. Myanmar is a new country record for N. iae, Harpiocephalus harpia (Temminck) is a new host record for N. iae and the preferred host is the Great Evening Bat, I. io.