Two new species of the genus Eotetranychus are described from the Huaping National Nature Reserve, Guangxi Zhuang Autonomous Region, China. Eotetranychus delphinus sp. nov. was collected from Schizophragma integrifolium Oliv. (Hydrangeaceae); E. enpeii sp. nov. was collected from Litsea cubeba (Lour.) Pers (Lauraceae). We also provide COI sequence data of these two new species: E. delphinus sp. nov. (GenBank accession numbers PP582994) and E. enpeii sp. nov. (GenBank accession numbers PP582995).