Two new species of oribatid mites of the genus Eremobelba (Oribatida, Eremobelbidae) collected from Gutianshan National Nature Reserve, Zhejiang, China were described: Eremobelba yinae sp. nov. and Eremobelba rotunda sp. nov.. A key to the known species of Eremobelba occur in China was provided.

Amblyomma multipunctum is a poorly known Neotropical tick (Acari, Ixodidae) described in 1899. It has been found associated with large mammals such as tapirs (Tapirus), and the Andean bear (Tremarctos ornatus). Males, females and nymphs have been described; however, the larvae of the species remain unknown. Valid records for A. multipunctum are few and limited to three countries in northwestern South America: Colombia, Ecuador, and Venezuela. In the present work, two partially engorged nymphs of A. multipunctum parasitizing the northern pudu, Pudu (Pudella) mephistophiles, were collected near to the Puracé National Natural Park, Department of Cauca, Colombia. Ticks were identified by morphology, with diagnostic characters such as scutum with multiple punctuations, two separate spurs in coxa I (the external being longer than the internal), a small triangular spur in coxae II–IV, spiracular plate triangular with rounded angles and a vestigial prolongation in article I of the palps. In addition, the taxonomical identity was validated using molecular evidence by PCR amplification of a fragment of the 16S rDNA gene, showing an identity percentage of 100% comparing with sequences in GenBank for A. multipunctum. This study reports the first association of A. multipunctum with P. mephistophiles, the extension of geographical distribution in Colombia, extending its elevational range by 644 m in the country, and contributing to the knowledge of the intraspecific variation of the tick nymph.

Two new species of the subgenus Pergalumna (Pergalumna)—P. (P.) araguaensis sp. nov. and P. (P.) bispinata sp. nov.—are described, based on adults collected from leaf litter in the Aragua State, Northern Venezuela. Pergalumna (P.) araguaensis differs from the related species P. (P.) espejillosensis by the morphology of the bothridial seta and rostrum, the location of the notogastral porose area Aa and solenidion on the leg tibia IV, the absence of the dorsosejugal porose area. Pergalumna (P.) bispinata differs from the related species P. (P.) montana by the body size, the morphology of head of the bothridial seta, the number of the notogastral porose areas, the presence of the median pore, the absence of the postanal porose area and striate ornamentation on the pteromorph.

A new species, Coprocarpais maximus Yao & Jin sp. nov., is described based on deutonymph and female samples from Hainan provinces, China. An identification key to the known species of Coprocarpais is provided.

The genus Mullederia (Acariformes: Stigmaeidae) are free-living predatory mites with six species known in the world. In this paper, we describe and illustrate a new species, Mullederia hainanensis sp. nov., from deciduous broad-leaved plants and evergreen broad-leaved plants in Hainan Province, China. Moreover, an identification key is provided for the known species of this genus.

Abrolophus boleticolus sp. nov. and Marantelophus kastamonuensis sp. nov. are described and illustrated from larvae collected from the wild, edible mushrooms, Boletus aereus (Boletaceae) and Marasmius oreades (Marasmiaceae), respectively, in Türkiye. Both of these erythraeid genera, which belong to the subfamily Abrolophinae, have comb-like seta on the palptarsus. In addition, Marantelophus viticolus, which was earlier synonymized with M. rudaensis, was determined to be a valid species.

Of the 44 species within the genus Dermacentor (Acari: Ixodidae) worldwide, nine are found in the Neotropics and four are endemic to this region. In Colombia, located in the northwestern corner of South America, there are two species of the genus: Dermacentor imitans restricted to the trans-Andean Pacific region, and Dermacentor nitens widely distributed in the country. Elsewhere, D. nitens is found in both the Nearctic and Neotropics, from southern USA to northern Argentina. In the Neotropics, D. nitens is widely distributed including records in Caribbean islands. Molecular analyses of D. nitens collected in the Amazon region of Colombia have shown genetic differences with specimens from other localities of the country, although morphological differences have not been explored. We performed morphological and molecular comparisons of individuals from two natural regions of Colombia: the Orinoquia, and the Amazon. The morphological and morphometric results show that females and males are grouped in two morphotypes: morphotype I from the Orinoquia region, and morphotype II from Leticia in the Amazon region. The molecular analyses supported the morphological results and showed the formation of two well-differentiated sister clades within D. nitens, with divergences between both morphotypes of 3–4% and 5–6% for the 16S rRNA and COI genes, respectively. Species delimitation analyzes and the Poisson Tree Processes model tentatively suggest the presence of two species within the sequences associated with D. nitens in Colombia. The first represented by the sequences from the Orinoquia region evaluated here, together with sequences from other regions of Colombia and other Neotropical countries such as Brazil, Panama, and French Guiana. The second is represented by the sequences of individuals from Leticia, in the Amazon region of Colombia. The results highlight that D. nitens might represent a species complex, but further sampling, experimental crosses, and studies at the level of complete genomes should be performed to obtain additional evidence for species recognition.

Two new species of the genus Eotetranychus are described from the Huaping National Nature Reserve, Guangxi Zhuang Autonomous Region, China. Eotetranychus delphinus sp. nov. was collected from Schizophragma integrifolium Oliv. (Hydrangeaceae); E. enpeii sp. nov. was collected from Litsea cubeba (Lour.) Pers (Lauraceae). We also provide COI sequence data of these two new species: E. delphinus sp. nov. (GenBank accession numbers PP582994) and E. enpeii sp. nov. (GenBank accession numbers PP582995).

Oribatid mites play a crucial role in nutrient cycling, litter decomposition, and humus formation in the ecosystem. Here, an Illumina HiSeq platform was used to sequence the complete mitochondrial (mt) genome of Ceratozetella imperatoria. The mt genome is a circular molecule of 14,229 bp (GenBank number: PP419053), and a total of 37 genes were described. The start codon in all 13 protein-coding genes (PCGs) included ATT, ATA, TTG, and ATG, the stop codon of all 13 protein-coding genes was TAA, TAG, and single T. The AT skew and GC skew were 0.153 and 0.263 observed across the whole mt genome of Ceratozetella imperatoria. Besides the negative AT skew in PCGs, rRNA and CR, positive AT skew and GC skew were detected in the remaining components. A phylogenetic tree of 29 mite species was constructed using PCGs by the maximum likelihood method. Ceratozetoidea and Oripodoidea were closely related and become sister group relationships among them.