Florida beggarweed is native to the Western Hemisphere but is naturalized around the world. During the last century, the mechanization of agriculture has transitioned Florida beggarweed from an important forage component to a weed of significance in the coastal plain of the southeast United States. This herbaceous annual is naturalized and found in fields and disturbed areas throughout the southern United States. The characteristics that made Florida beggarweed a good forage crop also make it a formidable weed. This review describes the importance of Florida beggarweed as a weed in the southern United States and the taxonomy of this species and details the distribution throughout the world and within the United States. The ecology of Florida beggarweed and its interactions with crop plants, insects, nematodes, and plant pathogens also are summarized. Finally, management of Florida beggarweed in agricultural systems using cultural practices and herbicides is reviewed.

Nomenclature: Florida beggarweed, Desmodium tortuosum (Schwartz) DC. DEDTO.

Tomato and pepper differ in their whole-plant tolerance to sulfonylurea (SU) herbicides rimsulfuron and halosulfuron despite both being members of the Solanaceae family. This study examined whether tomato's tolerance to SU herbicides rimsulfuron and halosulfuron was due to insensitivity of the target enzyme acetolactate synthase (ALS). Rimsulfuron and halosulfuron inhibited ALS from both tomato and pepper leaves. Enzyme inhibition and kinetic analyses showed that extractable ALS from tomato was more sensitive to rimsulfuron and halosulfuron than ALS from pepper. ALS from both species were inhibited with a mixed inhibition pattern. Thus, results indicate that enzyme insensitivity is not the reason why tomato is more tolerant than pepper to these herbicides. Tomato tolerance to rimsulfuron at the whole-plant level was reduced in the presence of terbufos, a known inhibitor of cytochrome P450 enzymes. Rimsulfuron applied at 0.018 and 0.035 kg ha⁻¹ with 1.1 kg ha⁻¹ of terbufos reduced tomato shoot weight 69 and 66%, respectively, compared with a 4 and 29% reduction when rimsulfuron was applied alone. The reduction of tomato tolerance to rimsulfuron by terbufos suggests that the sensitivity differences between these species may reflect their differences in SU herbicide metabolism.

Nomenclature: Halosulfuron; rimsulfuron; pepper, Capsicum annuum L. ‘X3R-Camelot’; tomato, Lycopersicon esculentum Mill. ‘FL 47’.

Broom and threadleaf snakeweed are major rangeland weeds in the western United States, and picloram is the major herbicide used for their management. Previous work has shown that these species are most susceptible to picloram applied in autumn or when precipitation is high and that differences in herbicide absorption and tissue sensitivity as measured by picloram-induced ethylene production do not fully explain variation in seasonal response. Therefore, the role of picloram metabolism in seasonal susceptibility to picloram was examined. Because snakeweed is characterized as highly genetically variable, picloram metabolism was evaluated monthly for 3 yr among populations from two species as well. Picloram metabolism was examined monthly for 3 yr among two populations of threadleaf and nine populations of broom snakeweed grown in a common garden. Metabolism ranged from 30 to 70% of picloram applied, and picloram was converted to two metabolites more polar than picloram regardless of species or population. Although metabolism was greatest in the year with the most precipitation, rate of metabolism was unrelated to precipitation received in the 7-d period before treatment. Application timing as defined by a given month or specific phenological stage was not related to the level of metabolism. We conclude that variation in picloram metabolism is not involved in differential susceptibility across season or population.

Nomenclature: Picloram; broom snakeweed, Gutierrezia sarothrae (Pursh) Britt. & Rusby GUESA; threadleaf snakeweed, Gutierrezia microcephala (DC.) Gray GUEMI.

Three herbicides were compared for their ability to reduce both carbon fixation and soil water depletion by yellow nutsedge in a growth chamber study. Whole-plant CO₂ exchange and water use were measured for 11 d after herbicide application. MSMA reduced carbon assimilation relative to the untreated control 1 d after treatment, and by 5 d after treatment respiration exceeded carbon assimilation during the photoperiod; however, MSMA had no significant effect on whole-plant water use during the measurement period. Halosulfuron reduced gross carbon assimilation to 30% of the pretreatment rate by the end of the experiment, but in contrast to MSMA it also strongly suppressed water use. Mesotrione never reduced carbon assimilation below 59% of the pretreatment rate and had no measurable effect on water use. Halosulfuron and MSMA reduced shoot regrowth to between 0 and 5% of the control, whereas mesotrione treatment allowed some 58% regrowth. These results indicate that whereas both MSMA and halosulfuron should provide effective control of yellow nutsedge, halosulfuron may be better able to rapidly suppress the weed's ability to compete for available soil water.

Nomenclature: Halosulfuron; mesotrione; MSMA; yellow nutsedge, Cyperus esculentus L. CYPES.

Germination of weed seed and time of emergence are greatly affected by temperature. The effects of temperature on seed germination of tumble pigweed, prostrate pigweed, smooth pigweed, Palmer amaranth, Powell amaranth, spiny amaranth, redroot pigweed, common waterhemp, and tall waterhemp were examined under constant and alternating temperature regimens at 5, 10, 15, 20, 25, 30, and 35 C. Averaged over all temperatures, alternating temperature regimens increased total germination of all species, except Powell amaranth, which germinated similarly under both constant and alternating temperatures. In addition, Powell amaranth seed exhibited the highest total germination across all temperatures compared with the other amaranth species. Prostrate pigweed seed demonstrated the lowest total germination. Optimal temperatures for maximum germination were greater than 20 C for all species, except prostrate pigweed. The alternating temperature regimen centering at 30 C was used to compare the germination rates of the nine species. Palmer amaranth and smooth pigweed attained complete germination on the first day. The rate of germination for these species was much more rapid than the other Amaranthus spp., which took 3 to 8 d to reach 50% germination.

Nomenclature: Common waterhemp, Amaranthus rudis Sauer AMATA; Palmer amaranth, Amaranthus palmeri S.Wats. AMAPA; Powell amaranth, Amaranthus powelli S.Wats. AMAPO; prostrate pigweed, Amaranthus blitoides S.Wats. AMABL; smooth pigweed, Amaranthus hybridus L. AMACH; spiny amaranth, Amaranthus spinosus L. AMASP; redroot pigweed, Amaranthus retroflexus L. AMARE; tall waterhemp, Amaranthus tuberculatus Sauer AMATU; tumble pigweed, Amaranthus albus L. AMAAL.

Field experiments were conducted to evaluate the potential of hyperspectral reflectance data collected with a hand-held spectroradiometer to discriminate soybean intermixed with pitted morningglory and weed-free soybean in conventional till and no-till plots containing rye, hairy vetch, or no cover crop residue. Pitted morningglory was in the cotyledon to six-leaf growth stage. Seven 50-nm spectral bands (one ultraviolet, two visible, four near-infrared) derived from each hyperspectral reflectance measurement were used as discrimination variables. Pitted morningglory plant size had more influence on discriminant capabilities than tillage or cover crop residue systems. Across all tillage and residue systems, discrimination accuracy was 71 to 95%, depending on the size of pitted morningglory plants at the time of data acquisition. The versatility of the seven 50-nm bands was tested by using a discriminant model developed for one experiment location to test discriminant capabilities for the other experiment, with discrimination accuracy across all tillage and residue systems of 55 to 73%, depending on pitted morningglory plant size.

Nomenclature:  Hairy vetch, Trifolium incarnatum L.; pitted morningglory, Ipomoea lacunosa L. IPOLA; rye, Secale cereale L.; soybean, Glycine max (L.) Merr. ‘Asgrow 4702RR’.

Field research was conducted to determine the potential of hyperspectral remote sensing for discriminating plots of soybean intermixed with pitted morningglory and weed-free soybean with similar and different proportions of vegetation ground cover. Hyperspectral data were collected using a handheld spectroradiometer when pitted morningglory was in the cotyledon to two-leaf, two- to four-leaf, and four- to six-leaf growth stages. Synthesized reflectance measurements containing equal and unequal proportions of reflectance from vegetation were obtained, and seven 50-nm spectral bands (one ultraviolet, two visible, and four near-infrared) derived from each hyperspectral reflectance measurement were used as discrimination variables to differentiate weed-free soybean and soybean intermixed with pitted morningglory. Discrimination accuracy was 93 to 100% regardless of pitted morningglory growth stage and whether equal or unequal proportions of reflectance from vegetation existed in weed-free soybean and soybean intermixed with pitted morningglory. Discrimination accuracy was 88 to 98% when using the discriminant model developed for one experiment to discriminate soybean intermixed with pitted morningglory and weed-free soybean plots of the other experiment. Reflectance in the near-infrared spectrum was higher for weed-free soybean compared with soybean intermixed with pitted morningglory, and this difference affected the ability to discriminate weed-free soybean from soybean intermixed with pitted morningglory.

Nomenclature: Pitted morningglory, Ipomoea lacunosa L. IPOLA; soybean, Glycine max (L.) Merr. ‘Asgrow 4702RR’.

Common ragweed and common cocklebur plants were collected at two sites each in Illinois, Minnesota, and Ohio to analyze intraspecific variability of the gene encoding acetolactate synthase (ALS). A 385-nucleotide fragment within the coding sequence of ALS was compared among 24 plants of each of these two species from the six locations. Common ragweed ALS was highly variable, with polymorphisms observed at 48 (12.5%) of the 385 nucleotides among the 24 plants. Despite the numerous nucleotide polymorphisms, only two inferred amino acid polymorphisms were identified. No apparent population structure was suggested by the ALS sequence data, indicating widespread gene flow consistent with the wind-pollinated nature of common ragweed. In contrast to common ragweed, no ALS polymorphisms were identified among the common cocklebur plants used in this study. As a basis for comparing the extremes observed between common ragweed and common cocklebur, ALS intraspecific variability also was investigated in 10 plants each of tall waterhemp and smooth pigweed. Normalized to the number of plants analyzed, the number of nucleotide polymorphisms for both tall waterhemp and smooth pigweed was greater than that in common cocklebur but less than that observed in common ragweed. Information on variability of herbicide target-site genes may be useful in predicting the likelihood for herbicide-resistance development. However, all four of the species investigated in this study have evolved resistance to ALS-inhibiting herbicides, despite the different levels of ALS variability observed.

Nomenclature: Common cocklebur, Xanthium strumarium L. XANST; Common ragweed, Ambrosia artemisiifolia L. AMBEL; smooth pigweed, Amaranthus hybridus L. AMACH; tall waterhemp, Amaranthus tuberculatus (Moq.) Sauer AMATU.

Field experiments were conducted in central Iowa to determine the growth of common waterhemp emerging after postemergence herbicide applications in soybean. Common waterhemp survival declined as emergence was delayed in relation to soybean. Ninety percent of plants emerging at approximately the same time as soybean survived, whereas only 13% of plants emerging approximately 50 d after planting (DAP) survived to maturity. Biomass accumulation declined rapidly as emergence was delayed in relation to soybean. Delaying emergence from 14 to 28 DAP resulted in a 50 to 80% reduction in shoot biomass. Common waterhemp emerging 50 DAP produced only 1 to 10% of the biomass of plants emerging at the same time as soybean. Plants emerging with soybean produced approximately 300,000 to 2.3 million seeds plant⁻¹ depending on the location. Fecundity of common waterhemp plants was closely related to biomass accumulation and declined rapidly with delayed emergence. Although common waterhemp emerging after the V4 stage of soybean (40 DAP) are unlikely to affect crop yield because of high mortality levels and reduced growth, these plants may contribute significant seeds to the soil seed bank.

Nomenclature: Common waterhemp, Amaranthus rudis Sauer AMATA; soybean, Glycine max (L.) Merr.

Biennial wormwood has become an important weed problem in the northern Great Plains, but little is known about its biology. Biennial wormwood seeds were collected from Fargo, ND, and Fergus Falls, MN, for field experiments in 1999 and 2000 to determine the influence of transplanting date on growth, biomass, and seed production. Seeds were seeded in a greenhouse every 2 wk, and seedling rosettes were transplanted to the field 2 wk after emergence from April 30 until September 15 to simulate season-long emergence. Weekly destructive subsampling started 2 wk after transplanting and ended on September 29 in both years. All seedlings that grew for at least 5 wk after transplanting produced flowers by mid- to late August of the same year. Late-transplanted seedlings with less than 5 wk of growth did not flower or survive the winter. Biennial wormwood biomass allocation patterns resemble those of an annual species, with about 15% of the total dry weight allocated to roots, 20% to stems, 25% to leaves, and 40% to flowers. Transplant date had a substantial influence on biomass partitioning. Seedlings transplanted early in the growing season produced more biomass and seed than late-season transplants. Biennial wormwood seedlings transplanted on April 30 produced over 435,000 seeds per plant, whereas seedlings transplanted on August 15 produced 500 to 3,000 seeds. Biennial wormwood was photoperiod sensitive and flowered when the day length was about 14 h or less, between August 18 and 25, in both years.

Nomenclature: Biennial wormwood, Artemisia biennis Willd. ARTBI.

Four experiments were conducted in central Iowa during 2001 and 2002 to determine the effects of weed emergence time and corn row spacing on common waterhemp growth and fecundity. Four common waterhemp emergence cohorts were established in each experiment and corresponded to the VE, V3, V5, and V8 stages of corn grown in rows spaced 38 and 76 cm apart. Common waterhemp mortality averaged 20, 56, 97, and 99% for the first, second, third, and fourth cohorts, respectively. Mean mature common waterhemp height for the first cohort was 140 cm, whereas plants emerging at the V8 corn stage were only 5 cm tall. Biomass of the first cohort was 20% less in 38-cm rows than in 76-cm rows, but later cohorts were not affected by row spacing. Biomass and seed production of waterhemp emerging at the V3, V5, and V8 corn stages decreased 80, 97, and 99%, respectively in comparison with the first cohort. Overall results indicate that common waterhemp biomass, survival, and fecundity decline sharply with delayed emergence relative to corn, but weeds emerging at or before the V5 corn stage may still contribute significantly to the seed bank.

Nomenclature: Common waterhemp, Amaranthus rudis Sauer AMATA; corn, Zea mays L.

Small broomrape, a holoparasitic weed, was recently introduced to the Pacific Northwest and contaminates a limited number of red clover fields in Oregon. Greenhouse and field studies were conducted to evaluate small broomrape response to common crop and weed species in the Pacific Northwest, and to evaluate a quick-screening method for plant species response to the parasite. In greenhouse studies, plants were grown in a hydroponic polyethylene bag system to allow for continuous visibility of their roots and monitoring of small broomrape seed germination and tubercle development. Results of the greenhouse study were validated in a field contaminated with small broomrape. In the greenhouse polyethylene bag study, small broomrape germinated and tubercles developed on alfalfa, arrowleaf clover, carrot, celery, crimson clover, lettuce, prickly lettuce, red clover, spotted catsear, subterranean clover, white clover, and wild carrot. Small broomrape germinated but did not develop tubercles when grown with barley, birdsfoot trefoil, common vetch, creeping bentgrass, cucumber, field corn, red fescue, flax, Italian ryegrass, nasturtium, oats, orchardgrass, perennial ryegrass, snap bean, sugar pea, sunflower, sweet corn, tall fescue, tomato, and wheat. In the field study, tubercles developed on alfalfa, arrowleaf clover, common vetch, crimson clover, red clover, subterranean clover, and white clover, but small broomrape flower stalks did not emerge in arrowleaf clover or crimson clover. Common vetch was a host for small broomrape in the field study but not in the hydroponic polyethylene bag system, thus, caution is warranted in using the quick screening method to elucidate potential small broomrape host species.

Nomenclature: Curly dock, Rumex crispus L. RUMCR; prickly lettuce, Lactuca serriola L. LACSE; spotted catsear, Hypochaeris radicata L. HYPRA; wild carrot, Daucus carota L. DAUCA; alfalfa, Medicago sativa L. MEDSA; arrowleaf clover, Trifolium vesciculosum Savi. TRFVE; barley, Hordeum vulgare L. HORVX; birdsfoot trefoil, Lotus corniculatus L. LOTCO; carrot, Daucus carota L. DAUCS; celery, Apium graveolens L. AOUGD; common vetch, Vicia sativa L. VICSA; creeping bentgrass, Agrostis stolonifera L. AGSTE; crimson clover, Trifolium incarnatum L. TRFIN; cucumber, Cucumis sativus L. CUMSA; field corn, Zea mays L. ZEAMA; flax, Linum usitatissimum L. LIUUT; Italian ryegrass, Lolium multiflorum Lam. LOLMU; lettuce, Lactuca sativa L. LACSA; nasturtium, Tropaeolum majus L. TOPMA; oats, Avena sativa L. AVESA; orchardgrass, Dactylis glomerata L. DACGL; perennial ryegrass, Lolium perenne L. LOLPE; red clover, Trifolium pratense L. TRFPR; red fescue, Festuca rubra L. FESRU; small broomrape, Orobanche minor Sm. ORAMI;

snap bean, Phaseolus vulgaris L. PHSVN; subterranean clover, Trifolium subterraneum L. TRFSU; sugar pea, Pisium sativum L. PIBST; sunflower, Helianthus annuus L. HELAN

Experiments were conducted in environmental chambers to the evaluate effects of photoperiod and temperature on Florida betony growth and development. Plants were exposed to two photoperiods, short day (9 h) and long day (9 3 h night interruption), and three day/night temperature regimes, 18/14, 22/18, and 26/22 C. After 10 wk of growth, shoot length and weight were 3.4 and 3.5 times greater, respectively, in the long-day photoperiod and with the 26 and 22 than with the 22 and 18 C day and night temperature regime, respectively. Shoot number, however, was greatest in the short-day photoperiod and at a lower temperature of 22/18 C. Shoot number in long day 22/18 C and 26/22 C environments increased asymptotically. No difference in root weight was observed between long- and short-day environments, but root weight increased with increasing temperature. Flowering and tuber production only occurred in long-day environments, with greater production of both at higher temperatures. Results provide a general framework for understanding Florida betony growth and development characteristics in the field and provide insights that should be considered in developing control strategies.

Nomenclature: Florida betony, Stachys floridana Shuttlew.

The development of optimal weed management strategies that rely, in part, on crop interference will require an understanding of how weeds compensate for limitations in above- and belowground resources. Trade-offs in the leaf morphology and biomass partitioning of rice and late watergrass were investigated under glasshouse conditions in 1999 and 2000. Both species responded to shade with increased height, reduced biomass, greater partitioning of biomass to leaves, and greater leaf area ratios. At the lowest light level (18% sunlight), plants of both species showed little response to nitrogen (N). However, height, tillers, biomass, and leaf area increased for plants grown at 50% and full sunlight as N increased from 0 to 224 kg N ha⁻¹. Late watergrass exhibited more plasticity in specific leaf area and root weight ratio than rice in response to shade. This plasticity contributed to the ability of late watergrass to maintain a higher percent of its tillers and total dry weight than rice when sunlight was reduced by 50%. These results support the hypothesis that except at low light levels, limited N further reduces the growth of shaded late watergrass plants. Thus, weed management strategies that limit the plasticity of late watergrass by manipulating light and N availability are likely to be more effective than strategies that rely on manipulating a single resource.

Nomenclature: Late watergrass, Echinochloa phyllopogon (Stapf) Koss ECHPH; rice, Oryza sativa L.

Interseeded cover crops have the potential to maintain and improve soil quality, reduce the incidence of insect pests, and suppress weeds in vegetable production systems. However, the successful use of interseeded cover crops has been limited by their tendency to either inadequately suppress weeds or suppress both weeds and the crop. We hypothesized that in irrigated broccoli production, winter rye could suppress annual weeds through rapid emergence and shading, without adversely affecting the taller transplanted broccoli crop. In field experiments conducted in New York from 1999–2001, broccoli was cultivated at 0, 10, or 10 and 20 d after broccoli transplanting (DAT), with or without rye at the final cultivation. Rye interseeded at 0 DAT suppressed weeds and improved yields relative to unweeded controls but resulted in broccoli yield losses relative to weed-free controls in 2 of 3 years. Rye seeded at either 10 or 20 DAT did not reduce broccoli yields but had little effect on weeds for a given level of cultivation and resulted in Powell amaranth seed production of up to 28,000 seeds m⁻². Rye interseeded at 0 DAT reduced light availability to weeds in 2000 but not in 2001 when Powell amaranth avoided shading from rye through rapid emergence and vertical growth. In greenhouse pot experiments, low temperatures for 7 d after seeding delayed the emergence of Powell amaranth by 3 d relative to rye and increased the suppression of Powell amaranth by rye from 61 to 85%. Our results suggest that winter rye may be more successfully integrated into broccoli production (1) when sown at higher densities, (2) in locations or seasons (e.g., spring) with lower initial temperatures, and (3) in combination with other weed management tools.

Nomenclature: Powell amaranth = green pigweed, Amaranthus powellii S. Wats. AMAPO; broccoli, Brassica oleracea L. var. italica PLENCK ‘marathon’; winter rye, Secale cereale L.

Differences in plant community composition have been attributed to abiotic field characteristics, crop type, localized predation, farm implement traffic, and natural dispersal mechanisms. Nitrogen (N) fertilizer rates and herbicides also are known to influence weed community structure, although their interaction has not been reported in the literature. A growth room experiment was conducted using three weed species (green foxtail, redroot pigweed, and velvetleaf) and five herbicides (nicosulfuron, atrazine, glufosinate, glyphosate, and mesotrione) differing in their mode of action and efficacy to the selected species. The experiment was conducted in growth chambers with two levels of N fertilization (low: 0.7 mM N and high: 7.7 mM N). Weeds were grown to the two- to five-leaf stage (depending on species), treated with the appropriate herbicide, and harvested approximately 2 wk after treatment. The herbicide dose at which a 50% reduction in biomass occurred (GR₅₀) was determined using log-logistic analysis. Herbicide susceptibility of the different weed species was influenced by N level. Green foxtail grown under low N required approximately six times the dose of nicosulfuron compared with plants grown under high N. Similarly, higher doses of nicosulfuron, glufosinate, mesotrione, and glyphosate were required to achieve a 50% reduction in redroot pigweed biomass grown under low N. In contrast, N did not influence the efficacy of mesotrione, glufosinate, or atrazine when applied to velvetleaf. This indicated specificity among herbicide–species combinations. Differences in herbicide efficacy resulting from soil N levels may alter weed community structure and may potentially explain possible weed control failures on farm fields.

Nomenclature: Atrazine; glufosinate; glyphosate; mesotrione; nicosulfuron; green foxtail, Setaria viridis (L.) Beauv. SETVI; redroot pigweed, Amaranthus retroflexus L. AMARE; velvetleaf, Abutilon theophrasti Medic. ABUTH.

The effects of a gall mite (Aceria malherbae) and sublethal doses of either 2,4-DB or glyphosate on field bindweed growth were evaluated under laboratory conditions. Mite feeding reduced field bindweed shoot biomass 37 to 48% and root biomass 46 to 50%. 2,4-DB at 0.07 to 0.14 kg ae ha⁻¹ or glyphosate at 0.14 to 0.28 kg ai ha⁻¹ reduced field bindweed root biomass 25 to 52%. Combining A. malherbae feeding with either 2,4-DB or glyphosate application reduced root biomass of field bindweed plants more than mites or either herbicide alone. Live A. malherbae were present on field bindweed 3 wk after treatment with either herbicide. Combination of A. malherbae with sublethal herbicide doses may allow for field bindweed suppression while reducing potential herbicide injury to crops and maintaining A. malherbae populations.

Nomenclature: 2,4-DB; glyphosate; field bindweed, Convolvulus arvensis L. CONAR.

Leaf cytoarchitecture was evaluated by light microscopy and scanning electron microscopy, and cell viability was monitored by fluorescence after treatment of velvetleaf with defined concentrations and droplet sizes of formulated glyphosate and blended tallowamine surfactant. In response to droplets of formulated glyphosate larger than in field sprays but useful for studying structural change, we observe that the leaf epidermis thins and flattens within 1.5 h, the epidermal, mesophyll, and vascular cells at the contact site exhibit localized cytolysis by 6 h, and cytolysis and pycnosis remain restricted to the contact site at 24 h. Using endogeneous fluorescence as a marker for nonviable cells, it was determined that cellular changes are directly correlated with droplet size and that the changes are minimal after exposure to spray sizes and concentrations of formulated glyphosate and blended tallowamine typically used in the field. The results show that, at field use concentrations, the effect of formulated glyphosate and blended tallowamine on leaf cytoarchitecture is modest and localized but sufficient to allow herbicide entry.

Nomenclature: Glyphosate; velvetleaf, Abutilon theophrasti Medicus ABUTH.

Clomazone has been successfully used for weed control in rice, but crop injury is a potential problem on light-textured soils. Experiments were conducted to determine the effect of soil characteristics and water potential on plant-available clomazone and rice injury. A centrifugal double-tube technique was used to determine plant-available concentration in soil solution (ACSS), total amount available in soil solution (TASS), and K_d values for clomazone on four soils at four water potentials. A rice bioassay was conducted parallel to the plant-available study to correlate biological availability to ACSS, TASS, and K_d. TASS was significantly different in all soils. The order of increasing TASS for the soils studied was Morey < Edna < Nada < Crowley, which correlated well with soil characteristics. The order of increasing TASS after equilibrium was − 90 < − 75 < − 33 < 0 kPa. TASS values at 0 kPa were greater than two times the TASS values at − 90 kPa. It appears that severe rice injury from clomazone on these soils could occur if TASS > 110 ng g⁻¹ and K_d < 1.1 ml g⁻¹. We propose that the double-tube technique provides a more accurate estimate of available herbicide because the solution–soil ratios are < 0.33:1 and would be more representative of a plant root–herbicide relationship. This technique or some variation possibly could be further developed such that clomazone rates could be more clearly defined particularly on lighter-textured soils. TASS may be a better predictor of plant-available herbicide than ACSS when evaluating moderately to highly water-soluble herbicides in a nonsaturated soil environment.

Nomenclature: Clomazone.