Broomrape species (Orobanche spp. and Phelipanche spp.) pose a tremendous threat to economically important crops in Iran; however, there is little information on the country's major broomrape species, their distribution and host ranges, and the subsequent crop losses they cause. The literature from Iran describes Egyptian broomrape (Orobanche aegyptiaca Pers.), hemp broomrape (Orobanche ramosa L.), Phelipanche nana (Reut.) Beck, and Orobanche cumana Wallr. as the major species of broomrape infesting agricultural fields. Solanaceae crops are among the most susceptible crops to infestation by broomrape in the country, and farmers are either shifting production to crops with lesser value or abandoning crop production entirely. Among infested crops, tomato (Solanum lycopersicum L.) is the most susceptible and widely infested, resulting in annual crop yield reductions of up to 40%. Chick pea (Cicer arietinum L.) and canola (Brassica napus L.) are also highly susceptible to broomrape infestation. Current control methods (physical, agronomical, biological, and chemical) have not proven to be effective or economic. The microscopic size of broomrape seeds and the presence of a wide range of host plants (weeds and crops) have promoted the spread of broomrape to new areas of Iran. Hence, the familiarization of farmers with the biology of broomrape would be highly effective in finding suitable control measures and in preventing its further distribution.

Protoporphyrinogen oxidase (PPO) is an important target for discovering new herbicides that interfere with the synthesis of porphyrin. To discover new PPO inhibitors with improved biological activity, a series of new diphenyl ethers containing tetrahydrophthalimide were designed and synthesized. Among them, J6.1 (IC₅₀ = 4.7 nM) and J6.3 (IC₅₀ = 30.0 nM) show higher maize (Zea mays L.) PPO inhibitory activity than the commercial herbicides oxyfluorfen (IC₅₀ = 117.9 nM) and flumioxazin (IC₅₀ = 157.1 nM). The greenhouse herbicidal activity of J6.3 is comparable to that of oxyfluorfen, and it is greater than that of flumioxazin. Even at a dose of 300 g ai ha^–1, cotton (Gossypium hirsutum L.) and peanut (Arachis hypogaea L.) show greater tolerance to J6.3, suggesting that J6.3 could be used for further development of new herbicide candidates in those fields. In addition, molecular docking has been used to further study the mechanism of action of J6.3. The results show that the introduction of a nitro group and tetrahydrophthalimide into the diphenyl ether structure is beneficial to biological activity.

The growth response of annual sowthistle (Sonchus oleraceus L.) to anticipated future climate conditions is currently unknown, and thus two parallel studies were conducted dealing with glyphosate-resistant (GR) and glyphosate-susceptible (GS) biotypes of S. oleraceus. The glyphosate efficacy study was conducted using different doses of glyphosate (0 [control], 180, 360, 720 [recommended dose], and 1,440 g ae ha^–1) at two different moisture levels (well-watered and water-stressed conditions). In the second study, the growth and seed production of these biotypes were studied under different atmospheric carbon dioxide (CO₂) concentrations (450 and 750 ppm) and under well-watered (100% field capacity) and water-stressed (50% field capacity) conditions. Results showed that the GR biotype survived (>68%) at 1,440 g ha^–1, but for the GS biotype, no plant survived, and both biotypes were slightly (<10%) affected by moisture regimes. In the elevated CO₂ condition, the GS biotype plants were >38% taller and produced >44%, >18%, and >21% more leaves, buds, and seeds, respectively, compared with the ambient CO₂ concentration under both moisture regimes. The biomass also increased by 27% in comparison with the ambient CO₂ concentration. For the GR biotype, plants at the elevated CO₂ level, while they also grew 38% taller in comparison with the ambient CO₂ concentration, the numbers of leaves, buds, and seeds and biomass were not affected by this increase in CO₂. Results showed that there were minimal changes in response to glyphosate for GR and GS biotypes of S. oleraceus with or without moisture stress. Our study suggests that future climate change with elevated CO₂ levels can affect the response of S. oleraceus to glyphosate, and such knowledge will be helpful for weed management in the future.

Palmer amaranth (Amaranthus palmeri S. Watson) populations resistant to acetolactate synthase (ALS)-inhibiting herbicides and glyphosate are fairly common throughout the state of North Carolina (NC). This has led farm managers to rely more heavily on herbicides with other sites of action (SOA) for A. palmeri control, especially protoporphyrinogen oxidase and glutamine synthetase inhibitors. In the fall of 2016, seeds from A. palmeri populations were collected from the NC Coastal Plain, the state's most prominent agricultural region. In separate experiments, plants with 2 to 4 leaves from the 110 populations were treated with field use rates of glyphosate, glufosinate-ammonium, fomesafen, mesotrione, or thifensulfuron-methyl. Percent visible control and survival were evaluated 3 wk after treatment. Survival frequencies were highest following glyphosate (99%) or thifensulfuron-methyl (96%) treatment. Known mutations conferring resistance to ALS inhibitors were found in populations surviving thifensulfuron-methyl application (Ala-122-Ser, Pro-197-Ser, Trp-574-Leu, and/or Ser-653-Asn), in addition to a new mutation (Ala-282-Asp) that requires further investigation. Forty-two populations had survivors after mesotrione application, with one population having 17% survival. Four populations survived fomesafen treatment, while none survived glufosinate. Dose–response studies showed an increase in fomesafen needed to kill 50% of two populations (LD₅₀); however, these rates were far below the field use rate (less than 5 g ha^–1). In two populations following mesotrione dose–response studies, a 2.4- to 3.3-fold increase was noted, with LD₉₀ values approaching the field use rate (72.8 and 89.8 g ha^–1). Screening of the progeny of individuals surviving mesotrione confirmed the presence of resistance alleles, as there were a higher number of survivors at the 1X rate compared with the parent population, confirming resistance to mesotrione. These data suggest A. palmeri resistant to chemistries other than glyphosate and thifensulfuron-methyl are present in NC, which highlights the need for weed management approaches to mitigate the evolution and spread of herbicide-resistant populations.

The transgenic Liberty Link^® (LL) soybean is tolerant to glufosinate, conferred by the enzyme phosphinothricin acetyltransferase (PAT), which is encoded by the pat gene from Streptomyces viridochromogenes. Because symptoms of injury can be observed in soybean [Glycine max (L.) Merr.] plants in some situations, this study evaluated the effects of rates of glufosinate on agronomic performance; quality of LL soybean seeds; and the ammonia, glufosinate, and N-acetyl-L-glufosinate concentration (NAG) in soybeans with and without the pat gene after application of increasing glufosinate rates. Field and greenhouse experiments were conducted; the first evaluated the selectivity of glufosinate in LL soybeans, and the second evaluated the metabolic changes in soybeans with (LL) and without (RR2) the pat gene, after application of glufosinate. For fieldwork, application of glufosinate at rates up to four times the maximum recommended caused initial injury symptoms (up to 38.5%) in LL soybean plants. However, no negative effect was found on seed quality and agronomic performance of LL plants, including yield. This shows the selectivity of glufosinate promoted by pat gene insertion for application in POST (V4), in LL soybean. For the greenhouse experiment, it was concluded that the LL soybean plants presented high glufosinate metabolism, lower ammonia concentration, and no reduction in dry matter, in comparison with RR2 soybean, after application of high rates of glufosinate.

In Australia, glyphosate is widely used in glyphosate-tolerant crops and fallows to control weeds such as common sowthistle (Sonchus oleraceus L.). It has been hypothesized that glyphosate at sublethal doses, as a consequence of herbicide drift, may have a stimulatory effect on S. oleraceus growth. In 2017, pot trials were conducted to evaluate the effect of low doses of glyphosate on growth and seed production of this weed at the Weed Science Screenhouse Facility at the University of Queensland, Australia. At the 4- to 5-leaf stage (3-wk-old rosette), plants were treated with low doses of glyphosate (0 [control], 5, 10, 20, 40, 80, and 800 g ae ha^–1), and their responses were recorded until plant maturity. The study was repeated after completion of the first experimental run. An additional glyphosate dose (2.5 g ha^–1) was added in the second run. The low doses of glyphosate (<40 g ha^–1) caused a significant increase in S. oleraceus plant height and number of leaves compared with the no-glyphosate treatment. The highest stimulatory effect was observed at 5 g ha^–1. At 5 g ha^–1 glyphosate, S. oleraceus seed production increased by 154% and 101% in the first and second experimental runs, respectively, compared with the no-glyphosate treatment. The results of this study suggest that the sublethal doses of glyphosate produced hormetic effects on growth and seed production of S. oleraceus that changed the dynamics of weed–crop competition.

Dwarf amaranth (Amaranthus macrocarpus Benth.) is a problematic broadleaf weed in many crops in Australia; however, no information is available on the germination ecology of this species. Seeds from two populations of this species were collected from Hillston, NSW, Australia (D-P-01), and Yandilla, QLD, Australia (D-P-02). Seeds were germinated at a range of constant (20 to 45 C) and alternating temperatures (30/20, 35/25, 40/30, and 45/35 C day/night). For the constant temperature treatments, the highest germination occurred at 35 C for D-P-01 (89%) and D-P-02 (82%). Germination was higher at the alternating day/night temperature of 40/30 C for both populations D-P-01 (91%) and D-P-02 (85%). Seed germination of both populations was stimulated by light, which indicates a great amount of emergence of A. macrocarpus can occur on bare ground such as crop seed beds. Results also revealed that this species tolerates a moderate level of salinity and can germinate in slightly alkaline soil conditions. The emergence of this species was highest (47%) for the seed buried at 0.5-cm depth in grey cracking alkaline soil compared with seed buried at the same depth in acidic red soils. These results suggest that soil inversion by tillage to bury weed seeds below their maximum emergence depth could serve as an important tool for managing A. macrocarpus. The results from this study will help in developing more sustainable and effective integrated weed management tactics for the control of this weed and weeds with similar responses in summer cropping systems.

Giant chickweed [Myosoton aquaticum (L.) Moench], a troublesome broadleaf weed species, is widespread in winter wheat (Triticum aestivum L.) fields in China. However, limited information is available on its germination and seedling emergence ecology. Thus, three M. aquaticum populations (JS, HN, and AH) from different geographic regions were studied under laboratory conditions to determine the effects of different environmental factors on germination or seedling emergence. The seeds germinated at a relatively wide constant temperature range of 5 to 25 C; however, the optimal temperature for germination varied among the populations. Compared with constant temperatures, fluctuating temperatures with the same mean significantly improved the final germination of all populations. Light was not required for germination. The seeds germinated under a wide pH range of 3 to 10, and the optimum pH was 6 to 7, with a final germination percentage of 81% to 95%. The seeds of all populations showed similar sensitivities to osmotic potential and similar high tolerances of saline conditions. The seedling emergence of all populations decreased with increasing burial depth, and no emergence was observed when the seeds were buried more than 3 cm, but the AH population showed higher sensitivity to burial depth than the others. The results generated from this study will contribute to the development of integrated M. aquaticum management strategies for winter wheat fields in China, and deep plowing and late sowing of autumn-sown wheat are suggested for managing M. aquaticum, as it showed lower germination at a low temperature and under relatively deep burial.

Wild radish (Raphanus raphanistrum L.) is a weed found globally in agricultural systems. The facultative winter annual nature of this plant and high genetic variability makes modeling its growth and phenology difficult. In the present study, R. raphanistrum natural seedbanks exhibited a biphasic pattern of emergence, with emergence peaks occurring in both fall and spring. Traditional sigmoidal models were inadequate to fit this pattern, regardless of the predictive environmental variable, and a corresponding biphasic model (sigmoidal + Weibull) was used to describe emergence based on the best parameters. Each best-fit chronological, thermal, and hydrothermal model accounted for at least 85% of the variation of the validation data. Observations on phenology progression from four cohorts were used to create a common model that described all cohorts adequately. Different phenological stages were described using chronological, thermal, hydrothermal, daylength-dependent thermal time, and daylength-dependent hydrothermal time. Integrating daylength and temperature into the models was important for predicting reproductive stages of R. raphanistrum.

The soil weed seedbank is the source of future weed infestations. Seed predation can result in a large number of seed losses, thus contributing to weed biocontrol. Earthworms are important predators of seeds and seedlings and affect seeds and seedling survival after gut passage. A study was conducted to assess the ability of Pheretima guillelmi (Kinberg) to ingest and digest the seeds and seedlings of 15 main farmland weed species. Pheretima guillelmi ingested the seeds and seedlings of each weed species tested. The percentages of seeds and seedlings ingested were 96.7% to 100% and 21.7% to 94.2%, respectively. Pheretima guillelmi showed greater ingestion of seeds than seedlings for each species and digested the seeds and seedlings of each weed species tested to varying extents. The percentages of seeds and seedlings digested were less than 15% irrespective of the weed species. Passage through the gut of P. guillelmi affected the survival of seeds and seedlings. The germination of large crabgrass [Digitaria sanguinalis (L.) Scop.], green foxtail [Setaria viridis (L.) P. Beauv.], goosegrass [Eleusine indica (L.) Gaertn.], Chinese sprangletop [Leptochloa chinensis (L.) Nees], Malabar sprangletop [Leptochloa fusca (L.) Kunth], redroot pigweed (Amaranthus retroflexus L.), common purslane (Portulaca oleracea L.), barnyardgrass [Echinochloa crus-galli (L.) P. Beauv.], and ricefield flatsedge (Cyperus iria L.) seeds egested by P. guillelmi decreased by 46%, 49%, 47%, 25%, 38%, 26%, 32%, 13%, and 15%, respectively, compared with their respective controls. In contrast to seed ingestion, ingestion of seedlings by P. guillelmi was fatal to individuals of all weed species; no seedlings survived passage through the gut. Our results indicate that predation of weed seeds and seedlings by P. guillelmi probably depletes the soil weed seedbank and that the introduction of P. guillelmi into fields is a potential strategy for weed biocontrol in farmland.

Weed management during spring crop production in eastern Washington presents many challenges. Many spring crops are weak competitors with weeds. In May of 2010 and 2011, two spring crop trials were initiated near Pullman, WA, to compare the relative competitiveness of barley (Hordeum vulgare L.), wheat (Triticum aestivum L.), lentil (Lens culinaris Medik.), and pea (Pisum sativum L.) using cultivated oat (Avena sativa L.) as a surrogate for wild oat (Avena fatua L.) competition. The experiment was arranged as a split-block split-plot design with four replications. One set of main plots included three oat density treatments (0, 63, and 127 plants m^–2), while a second set included each crop species. Crop species main plots were then split into subplots of two different seeding rates (recommended and doubled). Crop populations decreased as oat density increased and increased as crop seeding rate increased. As oat density increased, preharvest crop biomass decreased for all crops, while oat biomass and yield increased. Oat biomass and yield were greater in legume plots compared with cereal plots. Increasing oat density decreased yields for all crops, whereas doubling crop seeding rate increased yields for barley and wheat in 2010 and barley in 2011. Compared with legumes, cereals were taller, produced more biomass, and were more competitive with oat.

Some well-managed organic soils are known to have higher crop yield potential than conventionally managed soils due to the greater soil quality and the ability to tolerate weed competition. However, low available soil mineral N and P in some organic systems may mask such soil quality–related benefits. We hypothesize that when plant-available N and P are not limiting, tillage-based highly diverse organic crop rotations have less yield loss (better crop tolerance) due to weed competition and higher crop yields than no-till conventional systems with low-diversity rotations. A greenhouse study was carried out in Saskatoon, Canada, using long-term (18-yr) organically managed soils (ORG) and no-till conventional soils (CONV) with three crop rotation diversities (LOW, MEDIUM, and HIGH) to compare the crop tolerance to weed competition under standard soil nutrient management conditions and under excess supply of mineral N and P. Under fertilized conditions, crop biomass increased by 50% and 69% in ORG and CONV systems, respectively. Weed biomass was similar between ORG and CONV systems under nonfertilized conditions but was 14% greater in CONV when excessive N and P were supplied. Crop biomass loss (crop tolerance) was not different among cropping systems under excess fertilizer or under standard fertilizer levels. Even with greater weed biomass under fertilized conditions, the CONV system showed crop tolerance similar to that of the ORG system. Under nonfertilized conditions, the crop biomass yield was 43% lower in ORG compared with CONV, and even after mineral N and P were applied, ORG systems showed less (17%) crop biomass than CONV. Further, differences in crop tolerance were not identified among crop rotations under both fertilizer levels. Overall, this study revealed that there were no yield benefits or better crop tolerance to weed competition in organically managed soils compared with no-till conventional soils, even under nonlimiting soil macronutrient conditions.

A field experiment was conducted at the research farm of the University of Tehran to evaluate the effect of cover crops and herbicide (nicosulfuron) dosage on corn (Zea mays L.) productivity. Cover crops suppressed weed biomass and density during the fallow period; however, there was no significant effect on weed biomass or weed density in the subsequent corn crop. A mixture of cereal rye (Secale cereale L.) and hairy vetch (Vicia villosa Roth.) was the most weed-suppressive cover in the fallow period. Nicosulfuron applied at different amounts suppressed weed biomass and density in corn when compared with weedy plots. Application of 40 g ai ha^–1 of nicosulfuron was as effective on weed control as the recommended application. Cover crops did not affect corn growth; however, corn yield improved with herbicide application.

More than 80% of soybean [Glycine max (L.) Merr.] in Brazil is cultivated in no-till systems, and although cover crops benefit the soil, they may reduce the amount of residual herbicides reaching the soil, thereby decreasing herbicide efficacy. The objective of this study was to evaluate sulfentrazone applied alone, sequentially after glyphosate, and in a tank mixture with glyphosate before planting no-till soybean. Experiments were performed in two cover crop systems: (1) pearl millet [Pennisetum glaucum (L.) R. Br.] and (2) forage sorghum [Sorghum bicolor (L.) Moench ssp. bicolor]. The treatments tested were: glyphosate (720 g ae ha^–1) at 20 d before sowing (DBS) followed by sulfentrazone (600 g ai ha^–1) at 10 DBS; glyphosate + sulfentrazone (720 g ae ha^–1 + 600 g ai ha^–1) for cover crop desiccation at 10 DBS; and sulfentrazone alone at 10 DBS without a cover crop. The accumulation of straw was 31% greater using sorghum rather than pearl millet. In the sorghum system, the concentration of sulfentrazone at 0 to 10 cm was 57% less with sequential application and 92% less with the tank mixture compared with the treatment without cover crop straw at 1 d after application (DAA). The same occurred in the pearl millet system, where the reduction was 33% and 80% for the sequential application and tank mixture, respectively. The absence of a cover crop resulted in greater sulfentrazone concentrations in the top layer of the soil when compared with the sequential application or tank mixture. At 31 and 53 DAA, the concentration of sulfentrazone at 10 to 20 and 20 to 40 cm did not differ among treatments. Precipitation of 90 mm was enough to remove the herbicide from the cover crop straw at 31 DAA when using sequential application. An additional 90-mm precipitation was necessary to promote the same result when using the tank mixture.