Herbicide drift to sensitive crops can result in significant injury, yield loss, and even crop destruction. When pesticide drift is reported to the Georgia Department of Agriculture (GDA), tissue samples are collected and analyzed for residues. Seven field studies were conducted in 2020 and 2021 in cooperation with the GDA to evaluate the effect of (1) time interval between simulated drift event and sampling, (2) low-dose herbicide rates, and (3) the sample collection methods on detecting herbicide residues in cotton (Gossypium hirsutum L.) foliage. Simulated drift rates of 2,4-D, dicamba, and imazapyr were applied to non-tolerant cotton in the 8- to 9-leaf stage with plant samples collected at 7 or 21 d after treatment (DAT). During collection, plant sampling consisted of removing entire plants or removing new growth occurring after the 7-leaf stage. Visual cotton injury from 2,4-D reached 43% to 75% at 0.001 and 0.004 kg ae ha–¹, respectively; for dicamba, it was 9% to 41% at 0.003 or 0.014 kg ae ha–¹, respectively; and for imazapyr, it was 1% to 74% with 0.004 and 0.03 kg ae ha–¹ rates, respectively. Yield loss was observed with both rates of 2,4-D (11% to 51%) and with the high rate of imazapyr (52%); dicamba did not influence yield. Herbicide residues were detected in 88%, 88%, and 69% of samples collected from plants treated with 2,4-D, dicamba, and imazapyr, respectively, at 7 DAT compared with 25%, 16%, and 22% when samples were collected at 21 DAT, highlighting the importance of sampling quickly after a drift event. Although the interval between drift event and sampling, drift rate, and sampling method can all influence residue detection for 2,4-D, dicamba, and imazapyr, the factor with the greatest influence is the amount of time between drift and sample collection.