The chromosome numbers of six Alchemilla species from NE Anatolia, Turkey, belonging to A. sect. Alchemilla ser. Calycinae and ser. Elatae, respectively, are presented for the first time. Alchemilla ancerensis has 2n = 86–96, A. cimilensis 2n = 64–75, A. elevitensis 2n = 84–102, A. hemsinica 2n = 72–80, A. ikizdereensis 2n = 101–108, and A. kackarensis 2n = 66–76.