Lead (Pb) is a toxic element banned from fuel, paint and many other products in most developed countries. Nonetheless, it is still widely used in ammunition, including rifle bullets, and Pb-based bullets are almost universally used in Australia. For decades, poisoning from Pb shot (shotguns) has been recognised as a cause of disease in waterfowl and Pb shot has been subsequently banned for waterfowl hunting in many jurisdictions. However, the risks posed by Pb-based bullets (rifles) have not been similarly recognised in Australia. Pb-based rifle bullets frequently fragment, contaminating the tissue of shot animals. Consuming this Pb-contaminated tissue risks harmful Pb exposure and, thus, the health of wildlife scavengers (carrion eaters) and humans and their companion animals who consume harvested meat (game eaters). In Europe, North America and elsewhere, the environmental and human health risks of Pb-based bullets are widely recognised, and non-toxic alternatives (e.g. copper-based bullets) are increasingly being used. However, Australia has no comparable research despite widespread use of shooting, common scavenging by potentially susceptible wildlife species, and people regularly consuming shot meat. We conclude that Australia has its collective ‘head in the sand’ on this pressing worldwide One Health issue. We present the need for urgent research into this field in Australia.

Context. An understanding of the genetic stock structure of wide-ranging marine species is necessary for sound conservation management. Eretmochelys imbricata is Critically Endangered globally, but is among the least studied marine turtles. Reduced population sizes, its long-distance migratory nature between feeding and nesting habitats and poor understanding of its stock structure, biology and anthropogenic impact(s) pose challenges to developing effective conservation strategies for regional conspecifics.

Aims. Quantification of the population connectivity between specific feeding areas and regional nesting populations is needed for threat assessment and development of mitigation actions.

Methods. Here, we sequenced the mitochondrial DNA (mtDNA) of 91 immature and adult foraging E. imbricata individuals captured at the Howick Group of islands in the far-northern section of the Great Barrier Reef (nGBR), Queensland, Australia. We used a Bayesian mixed-stock analysis (MSA) approach to determine the contribution of nine regional genetically characterised breeding populations to this feeding aggregation.

Key results. The MSA estimated that a majority (83%; 95% CI = 70–92%) of feeding E. imbricata had originated from nesting beaches in the Bismarck–Solomon Sea region, whereas only 15% (95% CI = 6–25%) had originated from nGBR rookeries. International reproductive migrations were also corroborated by the return of 18 uniquely numbered titanium flipper tags that had been applied to E. imbricata found foraging in the Howick Group and had swum to rookeries within the Bismarck–Solomon Sea region. These 18 turtles represent 86% of all migration tag–recaptures from the Howick Group.

Conclusions. We postulate that recent increases in nesting populations within the Solomon Islands may be due to the high level of protection afforded to foraging turtles within the Great Barrier Reef.

Implications. An understanding of the connectivity between specific feeding areas and nesting populations is necessary to determine threats to animals over their entire life history and, therefore, allow the development of sound conservation management actions.

Context. Translocation as a tool for management of nuisance or ‘problem’ snakes near urban areas is currently used worldwide with limited success. Translocated snakes experience modified behaviours, spatial use and survivorship, and few studies have investigated the impacts of translocation within a metropolitan area.

Aims. In the present study, we investigated the impacts of translocation on the most commonly encountered snake in Perth Western Australia, the dugite (Pseudonaja affinis, Elapidae), by comparing the space use of resident and translocated snakes.

Methods. We captured 10 dugites and attached telemetry packages, composed of a radio-telemetry transmitter and global positioning system (GPS) data-logger, externally to their tails. Snakes were either released within 200 m of their initial capture sites (residents, n = 6) or moved to new unconnected habitat at least 3 km away (translocated, n = 4). Spatial-use data were analysed using general linear models to identify differences between resident and translocated dugites.

Key results. Translocation influenced space use of dugites and detrimentally affected their survivorship. Translocated snakes had larger activity ranges than did residents, and there was a trend towards travelling greater distances over time. Mortality for all snakes was high: 100% for translocated snakes, and 50% for residents.

Conclusions. Urban dugites face many threats, and snakes were negatively affected by translocation. The GPS technology we used did not improve the quality of the data over traditional radio-telemetry methods, owing to the cryptic nature of the snakes that spent much of their time under cover or underground.

Implications. These findings support the growing body of evidence that translocating ‘problem’ snakes is a not a humane method of animal management, and alternatives such as public education, may be more appropriate.

Context. Many mule deer (Odocoileus hemionus) populations in New Mexico have failed to recover from previous population declines, while some populations near urban areas have increased, resulting in more frequent human–wildlife conflicts. Translocations were used in an effort to simultaneously reduce an urban mule deer population and augment two low-density populations in south-western New Mexico, USA.

Aims. Because of insufficient monitoring, the efficacy of many ungulate translocations is unknown. Our goal was to monitor cause-specific mortality and 1 year post-release survival of mule deer translocated during 2013 and 2014. We compared survival rates of mule deer released with a hard- versus soft-release during the 2014 translocation.

Methods. We translocated 218 mule deer in 2013 and 2014 into the Peloncillo Mountains (PM) and San Francisco River Valley (SFRV); 106 adult female mule deer were fitted with telemetry collars to determine cause-specific mortality and estimate survival 1 year post-release. All deer were hard-released in 2013. In 2014, translocated mule deer were either held in a soft-release pen (0.81 ha) for approximately 3 weeks or hard-released into their new environment. We used a Kaplan–Meier approach to estimate survival of translocated mule deer at each release area and to compare survival of mule deer translocated using each release method (i.e. hard- versus soft-release).

Key results. In 2013–14, survival of hard-released deer in the PM was 0.627 (s.e. = 0.09), compared with 0.327 (s.e. = 0.10) in the SFRV. In 2014–15, survival of hard–released deer in the PM was 0.727 (s.e. = 0.13) and survival of soft-released deer was 0.786 (s.e. = 0.11). In the SFRV, survival of hard- and soft-released deer was 0.656 (s.e. = 0.14) and 0.50 (s.e. = 0.16), respectively. Causes of mortality were predation (51%), potential disease (9%; blue tongue or epizootic haemorrhagic disease), accident (5%), poaching (5%) and unknown (20%).

Conclusions. Translocations can be an effective management tool to augment populations of mule deer while reducing overabundant urban populations. Soft-released mule deer did not have higher survival than hard-released mule deer, although the length and conditions of the acclimation period were limited in our study.

Implications. Overabundant mule deer populations in urban areas may serve as sources of animals to bolster declining populations. Soft-release pens of smaller size and short period of acclimation did not influence survival.

Context. The abundance and distribution of mammalian species often change in response to environmental variability, losses or gains in suitable habitat and, in the case of pest species, control programs. Consequently, conventional distribution maps rapidly become out of date and fail to provide useful information for wildlife managers. For invasive brushtail possum populations in New Zealand, the main causes of change are control programs by central and local government agencies, and post-control recovery through recolonisation and in situ recruitment. Managers need to know current, and likely future, possum population levels relative to control targets to help assess success at preventing the spread of disease or for protecting indigenous species. Information on the outcomes of government-funded possum control needs to be readily available to members of the general public interested in issues such as conservation, disease management and animal welfare.

Aims. To produce dynamic, scalable maps of the current and predicted future distribution and abundance of possums in New Zealand, taking into account changes due to control, and to use recent visualisation technology to make this information accessible to managers and the general public for assessing control strategies at multiple spatial scales.

Methods. We updated an existing individual-based spatial model of possum population dynamics, extending it to represent all individuals in a national population of up to 40 million. In addition, we created a prototype interface for interactive web-based presentation of the model’s predictions.

Key results. The improved capability of the new model for assessing possum management at local-to-national scales provided for real-time, mapped updates and forecasts of the distribution and abundance of possums in New Zealand. The versatility of this platform was illustrated using scenarios for current and emerging issues in New Zealand. These are hypothetical incursions of possums, reinvasion of large areas cleared of possums, and impacts on animal welfare of national-scale management of possums as vectors of bovine tuberculosis (TB).

Conclusions. The new individual-based spatial model for possum populations in New Zealand demonstrated the utility of combining models of wildlife population dynamics with high-speed computing capability to provide up-to-date, easily accessible information on a species’ distribution and abundance. Applications include predictions for future changes in response to incursions, reinvasion and large-scale possum control. Similar models can be used for other species for which there are suitable demographic data, typically pest species, harvested species or species with a high conservation value.

Implications. Models such as the spatial model for possums in New Zealand can provide platforms for (1) real-time visualisation of wildlife distribution and abundance, (2) reporting and assessing progress towards achieving management goals at multiple scales, (3) use as a decision-support tool to scope potential changes in wildlife populations or simulate the outcomes of alternative management strategies, and (4) making information about pest control publicly available.

Context. Biological invasions have caused dramatic changes in native biodiversity and ecosystem function. Studies of genetic variation and evolutionary changes are useful for understanding population dynamics during biological invasions, and shed light on management, prevention and restoration strategies.

Aims. This study aimed to investigate the structure and genetic variability of American mink (Neovison vison), an invasive species in southern South America, introduced for fur farming in the 1930s.

Methods. Samples from 153 mink were obtained from 12 locations in southern Chile to sequence the mitochondrial DNA (mtDNA) control region and to genotype 11 polymorphic microsatellite loci.

Key results. The highest mtDNA diversity was detected in Puerto Cisnes, suggesting multiple introductions and/or the most probable area where mink was first introduced. The latter is also supported by microsatellite data, because a high percentage of individuals from different locations were assigned to this location. All other locations showed low or no mtDNA diversity, possibly due to founder effect. The results also indicate marked population structure, with three genetic clusters coincident with the main historical introduction points, with low dispersal among them.

Conclusions. The results suggest that control strategies for American mink in southern Chile should be concentrated on these three genetically differentiated management units, and particularly on source populations and locations with low effective population size and restricted connectivity.

Implications. Genetic approaches have been used for the management of numerous alien species worldwide. Recommendations delivered here for American mink control could also be implemented in other regions and for other invasive species with similar genetic diversity distribution and connectivity.

Context. Domestic cats (Felis catus) are efficient and abundant non-native predators, recently labelled as primary contributors to global biodiversity loss.

Aims. Specific research goals included determining the proportion of hunters, estimating hunting efficiency, identifying primary prey and examining predictors of kill rate and efficacy.

Methods. We investigated hunting of wildlife by stray cats living in managed outdoor colonies on a barrier island in the southeastern USA, and monitored 29 stray cats seasonally in 2014 and 2015 using Kittycam video cameras.

Key results. In total, 24 cats exhibited hunting behaviour and 18 captured prey. The estimated average daily predation rate from these successful hunters was 6.15 kills per 24-h period. Hunting effectiveness (percentage of capture attempts that translate to a kill) was an average of 44%. The most common type of prey captured was invertebrate (primarily Orthopteran and Hemipteran insects), followed by amphibians and reptiles. Eighty-three percent of kills occurred between dusk and dawn.

Conclusions. Colony location (near undeveloped island habitat) was related to higher kill rates. Cat sex and nocturnal hunting activity were related to greater hunting efficiency.

Implications. These results address the significant gap in knowledge about stray cat hunting activities, and raise conservation concerns for some groups of organisms (reptiles and amphibians) that have not been widely identified as vulnerable to cat predation.

Context. Pathogenic infections are an important consideration for the conservation of native species, but obtaining such data from wild populations can be expensive and difficult. Two pathogens have been implicated in the decline of some koala (Phascolarctos cinereus) populations: urogenital infection with Chlamydia pecorum and koala retrovirus subgroup A (KoRV-A). Pathogen data for a wild koala population of conservation importance in South Gippsland, Victoria are essentially absent.

Aims. This study uses non-invasive sampling of koala scats to provide prevalence and genotype data for C. pecorum and KoRV-A in the South Gippsland koala population, and compares pathogen prevalence between wild koalas and koalas in rescue shelters.

Methods. C. pecorum and KoRV-A provirus were detected by PCR of DNA isolated from scats collected in the field. Pathogen genetic variation was investigated using DNA sequencing of the C. pecorum ompA and KoRV-A env genes.

Key results. C. pecorum and KoRV-A were detected in 61% and 27% of wild South Gippsland individuals tested, respectively. KoRV-A infection tended to be higher in shelter koalas compared with wild koalas. In contrast with other Victorian koala populations sampled, greater pathogen diversity was present in South Gippsland.

Conclusions. In the South Gippsland koala population, C. pecorum is widespread and common whereas KoRV appears less prevalent than previously thought. Further work exploring the dynamics of these pathogens in South Gippsland koalas is warranted and may help inform future conservation strategies for this important population.

Implications. Non-invasive genetic sampling from scats is a powerful method for obtaining data regarding pathogen prevalence and diversity in wildlife. The use of non-invasive methods for the study of pathogens may help fill research gaps in a way that would be difficult or expensive to achieve using traditional methods.