28 phanerogams of various families, from the mountains of Gerania, Pateras, Kitheron, Pastra and Elikon (SE Sterea Ellas, Greece) are cytologically studied. The chromosome numbers of 13 taxa (Aristolochia microstoma, Asperula baenitzii, A. pulvinaris, A. rigidula, Centaurea subsericans, Conium divaricatum, Johrenia distans, Peucedanum vittijugum subsp. vittijugum, Scorzonera serpentinica, Thlaspi pindicum, Thymus parnassicus, Th. teucrioides subsp. candilicus, and Verbascum boissieri) are presented for the first time. In addition, Greek populations of 11 taxa are cytologically examined for the first time. Mitotic metaphase photomicrographs and/or karyograms are presented for the majority of taxa studied. Brief comments are given on the karyotype morphology, cytogeography and relationships of selected taxa.